COMPARATIVE ANALYSIS OF ASCARIDIAL PARASITIC CRUDE ANTIGEN BY SDS-PAGE AND UV ABSORBANCE TECHNIQUES | ||||
Veterinary Medical Journal (Giza) | ||||
Volume 42, Issue 1, January 1994, Page 41-45 PDF (1.54 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/vmjg.1994.372013 | ||||
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Authors | ||||
M MAHMOUD* 1; M SIAM2 | ||||
1Animal Health Research Inst., Dokki, Giza, Egypt. | ||||
2Fac. of Vet. Med., Cairo Unviersity, Egypt. | ||||
Abstract | ||||
Ascaridial nematodes are among the well-known parasites infesting fish, birds and mammals. Crude antigen was extracted from Amplicaecum larvae (AL3), Ascaridia galli (adult worm-AG) and Neoascaris vitulorum (adult worm-AV). The charactcrization of these parasitic nematodes revealed relative homologous polypeptide chains (MW 25.5 KDa) common to the three parasitic species, whereas the UV absorbance scan mode wave length (WL 200/550 nm) as related to the absorbance (AB) value zero-2 recorded an indicative frequency curve. The protein concentration in Amplicaecum, Ascaridia and Neoascaris figured up to 5.47 g%, 4.93 g% and 2.20 g%, respectively. | ||||
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