MYCOPLASMA AS CONTAMINANT OF CELL QULTURE WITH SPECIAL REFERENCE TO SOURCE OF INFECTION | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 40, Issue 2, April 1992, Page 43-47 PDF (1.21 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.1992.372191 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| LAILA EL-SHABINY* ; IKRAM KARIM; EL-NAKASHL S | ||||
| Animal Health Research Inst. Doki Giza. | ||||
| Abstract | ||||
| Nine tissue cultures, 7 media for virus propagation and 5 lots of bovine sera as enrichment were brought to the Mycoplasma research department and routinely examined for contamination with Mycoplasma Four M. hominis were isolated from cell cultures, 3 M. salivarium were isolated from culture media while bovine serum samples revealed 3 bovine mycoplasmas isolates, 2 of them were M. arginini and one as A. Laidlawii. Antibiotic sensitivity test for Mycoplasma isolates was applied against 8 antimicrobial agents using metabolic inhibition test (MIT) and growth inhibition test (GIT) and spectinomycin proved to be the most effective and was recommended for treatment of contaminated tissue cultures. A trial was made to investigate the source of contamination. Out of 10 laboratory personnel 7 (.70%) Mycoplasma salivanum were isolated from throat samples while 6 (60%) were isolated when they were allowed to talk in front of plates held 2 inches from the mouth. | ||||
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