Microbial Interdisciplinary Experiment: Unravelling the outcome of Lglutaminase Produced by Streptomycetes isolates | ||||
| Journal of Basic and Environmental Sciences | ||||
| Volume 10, Issue 4, October 2023, Page 114-129 PDF (372.72 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jbes.2023.372264 | ||||
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| Authors | ||||
| Mohamed O. Abdelmonem1; Mohamed E. El awady2; Mohamed I. El-khonezy3; Mervat G. Hassan1; Mona A. Kishk1 | ||||
| 1Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt. | ||||
| 2Microbial Biotechnology Department, Biotechnology Research Institute, National Research Centre, El-Buhouth St. 33, Dokki, Cairo, Egypt. | ||||
| 3Molecular Biology Department, National Research Centre, 33-El Bohouth St., Dokki, Giza, P.O.12622, Egypt. | ||||
| Abstract | ||||
| It is well known that Streptomyces produces beneficial pharmaceuticals, particularly antibiotics and anticancer agents. This investigation uncovered 34 randomly selected streptomycetes isolates in sediment, marine water, soil, and other habitats. Using a qualitative rapid plate assay to test for GluNase synthesis, 9 of them produced a significant amount of L-glutamine pink colour.The maximum levels of GluNase synthesis were obtained at a pH of 7.5, an incubation temperature of 40 °C, and the utilization of glucose and peptone as the carbon and nitrogen sources, respectively. | ||||
| Keywords | ||||
| Streptomycetes; GluNase; isolation; screening; optimiza | ||||
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