PREPARATION AND LABORATORY EVALUATION OF LIVE ATTENUATED INFECTIOUS BURSAL DISEASE (IBD) VIRUS VACCINE FROM RECENT EGYPTIAN ISOLATE PROPAGATED ON CHICKEN EMBRYO FIBROBLAST (CEF) CELL CULTURE. | ||||
Veterinary Medical Journal (Giza) | ||||
Volume 49, Issue 4, October 2001, Page 563-573 PDF (3.65 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/vmjg.2001.372275 | ||||
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Authors | ||||
H MADBOULY* 1; ENSAF M KHASHABAH2; NADIA M IBRAHIM2 | ||||
1Faculty of Veterinary Medicine, Cairo University, Beni-Suef branch. | ||||
2Veterinary Serum and Vaccine Research Institute, Abbassia. Cairo. | ||||
Abstract | ||||
Very virulent IBDV was propagated for 3 passages in SPF-ECE followed by 3 passages in young susceptible chicks then for 3 passages in SPF. ECE then for further 12 passages in SPF-ECE. The first passage of last propagation was further propagated for 3 passages on Vero cells and for 60 passages in CEF-cell culture. The passage 40 of propagated IBDV proved to be safe and highly immunogenic by virus neutralization test. It still elicited high neutralizing antibody titers (18 log2) for a stationary phase about 7 months (the end of the experiment) and high values of lymphocyte blastogenesis (0.560 versus control 0.03). Vaccinated chicks well protected against challenge with highly virulent IBDV after 3 weeks post-vaccination (PV). The prepared vaccine has superior potential immunogenic effect than commercial live mild, and intermediate plus (hot) vaccines. The vaccine is effective even when preserved for 8 months (the end of ex- - periment) at -20°C. | ||||
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