MODULATORY EFFECTS OF BUFFALO BASIC AND ACIDIC UTERINE LUMINAL PROTEINS ON PHAGOCYTIC AND BACTERICIDAL ACTIVITIES OF POLYMORPHONUCLEAR- AND MONONUCLEAR LEUKOCYTES IN VITRO
RAMADAN, A., ZAKI, A., HASSAN, H., HASHAD, M. (2001). MODULATORY EFFECTS OF BUFFALO BASIC AND ACIDIC UTERINE LUMINAL PROTEINS ON PHAGOCYTIC AND BACTERICIDAL ACTIVITIES OF POLYMORPHONUCLEAR- AND MONONUCLEAR LEUKOCYTES IN VITRO. EKB Journal Management System, 49(4), 609-622. doi: 10.21608/vmjg.2001.372282
A RAMADAN; A ZAKI; H HASSAN; M HASHAD. "MODULATORY EFFECTS OF BUFFALO BASIC AND ACIDIC UTERINE LUMINAL PROTEINS ON PHAGOCYTIC AND BACTERICIDAL ACTIVITIES OF POLYMORPHONUCLEAR- AND MONONUCLEAR LEUKOCYTES IN VITRO". EKB Journal Management System, 49, 4, 2001, 609-622. doi: 10.21608/vmjg.2001.372282
RAMADAN, A., ZAKI, A., HASSAN, H., HASHAD, M. (2001). 'MODULATORY EFFECTS OF BUFFALO BASIC AND ACIDIC UTERINE LUMINAL PROTEINS ON PHAGOCYTIC AND BACTERICIDAL ACTIVITIES OF POLYMORPHONUCLEAR- AND MONONUCLEAR LEUKOCYTES IN VITRO', EKB Journal Management System, 49(4), pp. 609-622. doi: 10.21608/vmjg.2001.372282
RAMADAN, A., ZAKI, A., HASSAN, H., HASHAD, M. MODULATORY EFFECTS OF BUFFALO BASIC AND ACIDIC UTERINE LUMINAL PROTEINS ON PHAGOCYTIC AND BACTERICIDAL ACTIVITIES OF POLYMORPHONUCLEAR- AND MONONUCLEAR LEUKOCYTES IN VITRO. EKB Journal Management System, 2001; 49(4): 609-622. doi: 10.21608/vmjg.2001.372282

MODULATORY EFFECTS OF BUFFALO BASIC AND ACIDIC UTERINE LUMINAL PROTEINS ON PHAGOCYTIC AND BACTERICIDAL ACTIVITIES OF POLYMORPHONUCLEAR- AND MONONUCLEAR LEUKOCYTES IN VITRO

Veterinary Medical Journal (Giza)
Volume 49, Issue 4, October 2001, Page 609-622  XML PDF (4.88 MB)
Document Type: Original Article
DOI: 10.21608/vmjg.2001.372282
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Authors
A RAMADAN* 1; A ZAKI1; H HASSAN2; M HASHAD2
1Immunobiology and Immunopharmacology Unit: Biology of Reproduction Department , Animal Reproduction Research Institute, P.O. Box 12556, Giza, Egypt
2Microbiology Dept. Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
Abstract
Potential immunological effects of basic and acid- ic uterine luminal proteins (ULP) collected from estrus- and luteal-phase buffaloes were studied in vitro. The phagocytic and microbcidal activities of PMN and macrophages, nitric oxide production by macrophages, and superoxide anion production by PMN were elucidated. Estrus-phase basic and acidic ULP collected from buffaloes enhanced phagocytic activity of PMN at all concentrations tested, whereas basic and acidic ULP of luteal- phase buffaloes suppressed phagocytic activity in a dose-dependent manner. The bactericidal activ- ity of buffalo PMN increased in response to basic ULP of estrus-phase buffaloes in comparison with basic luteal-phase proteins. Meanwhile, acidic ULP proteins of estrus-phase buffaloes showed significant increase in bactericidal activity only at 40, 80, and 150 μg/ml protein concentrations in contrast to acidic ULP of luteal-phase buffaloes. Nitric oxide production by macrophages in- creased in response to basic ULP of estrus-phase buffaloes, whereas this activity decreased when macrophages were treated with different concentrations of basic ULP of luteal-phase buffaloes. Only at high concentrations the acidic protein of estrus-phase buffaloes increased nitric oxide production by macrophages as compared with acidic proteins from luteal-phase buffaloes. Superoxide anion productions by neutrophils increased in response to basic and acidic ULP of estrus-phase buffaloes and decreased in response to acidic pro- teins from luteal-phase buffaloes. These data demonstrate that buffalo uterus contains immunologically active secretory proteins that can influence the natural defense barriers of the buffalo genital tract.
Keywords
buffalo; uterine proteins; estrous cy- cle; phagocytic cells
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