Ameliorative Effect of Vitamin C against Hydroxyurea-Induced Hepatic Toxicity in Male Rats | ||
| Egyptian Academic Journal of Biological Sciences, B. Zoology | ||
| Volume 16, Issue 2, December 2024, Pages 39-50 PDF (644.75 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsz.2024.374242 | ||
| Authors | ||
| Faiza Milad; Ebtesam Abdulhadi; Ghaliyah Aqeelah | ||
| Department of Zoology, Faculty of Sciences, University of Sirte, Sirte, Libya. | ||
| Abstract | ||
| Background and aim: Hydroxyurea (HU) possesses several characteristics of an ideal drug for various diseases, despite the reported side effects. The current study intends to search for the potential ameliorative effectiveness of vitamin C on hydroxyurea-induced hepatotoxicity in a rat model. Materials and methods: 24 adult male albino rats were split into four groups. Group I served as the control; Group II received 5 mg/kg bw of vitamin C; Group III received 100 mg/kg bw of HU; and Group IV received a combination of 5 mg/kg bw VC and 100 mg/kg bw HU, which were administered orally for 30 days to assess liver function tests, oxidative stress, histopathology, and the detection of DNA damage. Results: The results demonstrated a significant decline (p < 0.01) in the absolute organ weight of rats treated with HU. However, there was no significant contrast in the liver-relative condition. The rats exhibited elevated levels of AST, ALT, and MDA a reduction in albumin, total protein, SOD, CAT, and GSH. The group that received HU displayed signs of degeneration, hydropic deterioration, and fatty degeneration. A comet assay revealed an increase in tail length and DNA content in liver cells. There was no significant difference in olive tail moment but a significant decrease in % tailed, % DNA in the tail, and tail moment. Conclusion: The administration of VC with HU improved liver function tests, oxidative stress, and histopathology compared to the HU group. | ||
| Keywords | ||
| Hydroxyurea; Vitamin C; Hepatotoxicity; Histopathological; Genotoxicity | ||
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