Pathological Alterations in Lung and Liver of Male Mice Intoxicated with Benzo[a]pyrene: A time Dependent Study | ||
| Egyptian Journal of Veterinary Sciences | ||
| Article 9, Volume 56, Issue 9, September 2025, Pages 2073-2084 PDF (1.6 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2024.273105.1898 | ||
| Authors | ||
| Doaa Ahmed Zedan* 1; Fatma Mahmoud Darwish1; Iman Baker Mohamed2 | ||
| 1Pathology Department, Animal Health Research Institute (AHRI), Dokki, Giza, Egypt. | ||
| 2Pathology Department, Dean of Faculty of Veterinary Medicine, Cairo University, Giza, Egypt. | ||
| Abstract | ||
| This study aimed to investigate the pathological changes occurring in lungs and liver of male mice exposed to different doses of Benzo[a]pyrene (B[a]P) over varying durations. B[a]P is a potent carcinogenic polycyclic aromatic hydrocarbon commonly found in the environment, primarily adsorbed to particulate matter. Ninety mature mice were divided into three groups: a control negative group, a group injected with a single dose of 50 mg/kg bw of B[a] P, and a group injected with a single dose of 100 mg/kg bw of B[a] P. The experiment was conducted over several months, with ten mice from each group being sacrificed at 3, 6, and 9 months. Serum samples were collected for biochemical tests to assess liver function and tumor marker alpha-fetoprotein levels. Additionally, postmortem examinations were performed; lung and liver tissue specimens were collected for histopathological analysis. The results of the biochemical tests revealed a significant increase in Alanine aminotransferase (ALT), Aspartate aminotransferase (AST) and alpha-fetoprotein levels. Histopathological examination of the lung tissues showed a gradual initiation of mild hyperplastic proliferation in the bronchiolar epithelia at both half (50 mg/kg bw) and full (100 mg/kg bw) doses of B[a] P, with more pronounced changes observed in the full-dose groups at 6 and 9 months. At 9 months of full-dose treatment, a well-defined circumscribed tumor mass was detected. In the liver, cellular changes were observed at 3 months for both half and full doses of B[a]P, progressing to malignant changes at 6 months and ultimately leading to the development of hepatocellular adenocarcinoma at 9 months. | ||
| Keywords | ||
| Benzo[a]pyrene; lung; liver; histopathology | ||
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