Detection of Mold and Aflatoxin B1 in Mayonnaise Product from Egyptian Markets by HPLC | ||||
Journal of Applied Veterinary Sciences | ||||
Article 5, Volume 9, Issue 4, October 2024, Page 34-41 PDF (539.74 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/javs.2024.300420.1365 | ||||
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Authors | ||||
Neveen S.M. Soliman ![]() ![]() | ||||
1Food Hygiene Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt | ||||
2Food Hygiene Department, Animal Health Research Institute, Agriculture Research Center, Giza 12619, Egypt | ||||
3Microbiology Department, Central Laboratory of Residue Analysis of Pesticides and Heavy Metals in Food, Agriculture Research center, Dokki, Giza, Egypt | ||||
Abstract | ||||
Egyptian consumers' demand and preference for sauces like mayonnaise have increased lately. The processing and packaging techniques of such products may safeguard the consumer from bacterial hazards, although mold and/or mycotoxins are still expected hazards in such products.Therefore, the current investigation is intended to determine the incidence of mold and aflatoxin B1 in commercial mayonnaise sold in Egyptian markets.A total of thirty mayonnaise samples were arbitrarily gathered from Cairo and Giza governorates in order to determine the presence of mold using cultivation techniques and AFB1 using a low-cost high-recovery fluorescence detector (FLD) in combination with an easy-to-use, highly specific and specially developed High-Pressure Liquid Chromatography (HPLC) assay that adhered to green chemistry principles.About 53.33% of the examined samples were positive for AFB1, while mold couldn’t be detected in any of the examined samples. It was also discovered that 43.33% of AFB1 in total samples was below the maximum permitted threshold. Therefore, more attention is required from the authorities for continuous examination of such products that are present in the market for the incidence of chemical contamination with aflatoxin. | ||||
Keywords | ||||
Aflatoxin B1; Chemical hazard; Mayonnaise; Mold | ||||
References | ||||
ADEGBEYE, M. J., REDDY, P. R. K., CHILAKA, C. A., BALOGUN, O. B., ELGHANDOUR, M. M., RIVAS-CACERES, R. R., and SALEM, A. Z., 2020. Mycotoxin toxicity and residue in animal products: Prevalence, consumer exposure and reduction strategies–A review. Toxicon, 177, 96-108. https://doi.org/10.1016/j.toxicon.2020.01.007
ADEYEYE, S.A. 2016. Fungal mycotoxins in foods: A review. Cogent Food & Agriculture. https://doi.org/10.1080/23311932.2016.1213127
AGANOVIC, K., BINDRICH, U., and HEINZ, V., 2018. Ultra-high pressure homogenisation process for production of reduced fat mayonnaise with similar rheological characteristics as its full fat counterpart. Innovative Food Science and Emerging Technologies, 45, 208–214. https://doi.org/10.1016/j.ifset.2017.10.013
AKHILA, P.P. , SUNOOJ , K.V., AALIYA , B., NAVAF , M., SUDHEESH, C., SABU, S., SASIDHARAN , A., MIR, S. A., GEORGE, J., and KHANEGHAH, A. M., 2021. Application of electromagnetic radiations for decontamination of fungi and mycotoxins in food products: A comprehensive review. Trends in Food Science & Technology. Volume 114, Pages 399-409 https://doi.org/10.1016/j.tifs.2021.06.013
ALIZADEH , A . M. , HASHEMPOUR-BALTOR K , F., KHANEGHAH , A. M., and HOSSEINI, H., 2020. New perspective approaches in controlling fungi and mycotoxins in food using emerging and green technologies. Current Opinion in Food Science Vol. 9, 7-15 https://doi.org/10.1016/j.cofs.2020.12.006
ALSHANNAQ, A., and YU, J. H. 2017. Occurrence, toxicity, and analysis of major mycotoxins in food. International Journal of Environmental Research and Public Health 14 (6), 632 https://doi.org/10.3390/ijerph14060632
ALVAREZ-SABATEL, S., MARTÍNEZ DE MARAÑÓN, I., and ARBOLEYA, J. C., 2018. Impact of oil and inulin content on the stability and rheological properties of mayonnaise-like emulsions processed by rotor-stator homogenisation or high pressure homogenisation (HPH). Innovative Food Science and Emerging Technologies, 48, 195–203. https://doi.org/10.1016/j.ifset.2018.06.014
AMIRKHIZI, B.,AREFHOSSEINI, S. R., ANSARIN, M., and NEMATI, M., 2015. Aflatoxin B1 in eggs and chicken livers by dispersive liquid–liquid microextraction and HPLC. Food Addit. Contam. Part B 2015; 8(4), 245-249. https://doi.org/10.1080/19393210.2015.1067649
AOAC (OFFICIAL METHOD OF ANALYSIS), 1995. Aflatoxin in Milk and Cheese. TLC Method Chapter49, p. 30-32
ASIYE, A. 2019. Antibacterial and antifungal effect of achillea millefolium essential oil during shelf life of mayonnaise. December 2019 Food Science and Technology 13(4) https://doi.org/10.15673/fst.v13i4.1568
Bao, L., Mary, W. TRUCKSESS, and Kevin D. WHITE, 2010. Determination of aflatoxins B1, B2, G1, and G2 in olive oil, peanut oil, and sesame oil. Journal of AOAC International. 93 (3 )936-42. https://doi.org/10.1093/jaoac/93.3.936
BERTHILLER, F., MARAGOS, C.M., and DALL’ASTA, C., 2016. Introduction to masked mycotoxins. In Masked Mycotoxins in Food:Formation, Occurrence and Toxicological Relevance; DallAsta, C., Berthiller, F., Eds.; Royal Society of Chemistry: London, UK. Volume 24, pp. 1–13. https://doi.org/10.1039/9781782622574-00001
BHAT, R. K., and REDD,R. N., 2017. Challenges and issues concerning mycotoxins contamination in oil seeds and their edible oils: Updates from last decade. Food Chemistry Volume 215, Pages 425-437. https://doi.org/10.1016/j.foodchem.2016.07.161
BIBANI, N. M., KHIDHIR, Z. K., SHAKER, A. S., KIRKUKI, S. M., and ABDULATEEF, S. M., 2019. Analyses of mycotoxins in broiler’s local and imported feeds. Iraqi journal of veterinary sciences, 33(2), 267-271. http://dx.doi.org/10.33899/ijvs.2019.162994
CAPRIOTTI , A. L., CARUSO, G. , CAVALIERE, C. , FOGLIA, P. , SAMPERI, R., and LAGANÀ, A., 2012. Multiclass mycotoxin analysis in food, environmental and biological matrices with chromatography/mass spectrometry. Mass Spectrometry Reviews. volume 31(4) pages 466-503 https://doi.org/10.1002/mas.20351 CAST, 1989. Mycotoxins: Economic and Health Risks. Task Force Report no. 116. Council for Agricultural Science and Technology, Ames, Iowa, p. 91.
CHIVERO, P., GOHTANI, S., YOSHII, H., and NAKAMURA, A., 2016. Assessment of soy soluble polysaccharide, gum arabic and OSA-Starch as emulsifiers for mayonnaise-like emulsions. LWT-Food Science and Technology, 69, 59–66. https://doi.org/10.1016/j.lwt.2015.12.064
CIMBALO ,A., ALONSO-GARRIDO , M., FONT , G., and MANYES, L., 2020. Toxicity of mycotoxins in vivo on vertebrate organisms: A review. Food and Chemical Toxicology. Volume 137, 111161 https://doi.org/10.1016/j.fct.2020.111161
CLAEYS , L., CHIARA, R., KARL, DE R., HAYLEY, W., BEATRICE, F., MICHAEL, K., JIRI, Z., MARC, J. G., SARAH, DE S., MARTHE, DE B., and INGE, H., 2020. Mycotoxin exposure and human cancer risk: A systematic review of epidemiological studies. Compr Rev Food Sci Food Saf, 19(4): 1449-1464. https://doi.org/10.1111/1541-4337.12567
DA SILVA, E.O., ANA-PAULA, L., and ISABELLE, P. O., 2018. Mycotoxins and oxidative stress: Where are we? World Mycotoxin Journal 2018
DALIÉ, D. K. D., DESCHAMPS, A. M., and RICHARD, F., 2010. Lactic acid bacteria Potential for control of mold growth and mycotoxins. A review Food Control; 21:370–380. https://doi.org/10.1016/j.foodcont.2009.07.011
DE SOUZA, C.; KHANEGHAH, A. M., and OLIVEIR, C. A. F., 2021.The occurrence of aflatoxin M1 in industrial and traditional fermented milk: A systematic review study. Italian Journal of Food Science. volume 33 No. SP1 https://doi.org/10.15586/ijfs.v33iSP1.1982
EATON, D.L., and GALLAGHER, E.P., 1994. Mechanisms of Aflatoxin Carcinogenesis. Annual Review of Pharmacology and Toxicology, 34, 135-172. https://doi.org/10.1146/annurev.pa.34.040194.001031
EC, 1881/2006: Commission Regulation (EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs (Text with EEA relevance).
FERIAL M. ABU-SALEM and AZZA A. ABOU-ARAB, 2008. Chemical, microbiological and sensory evaluation of mayonnaise prepared from ostrich eggs. Grasas Y Aceites, 59(4) Octubre- Dicimbre, 352-360, ISSN: 0017-3495 vol 59 NO. 4 https://doi.org/10.3989/gya.2008.v59.i4.529
FERNÁNDEZ-CRUZ, M.L., MARÍA, L., FERNÁNDEZ- CRUZ, M., MANSILLA, L., and JOSÉ, L. T., 2010. Mycotoxins in fruits and their processed products: Analysis, occurrence and health implications. Journal of Advanced Research. Volume 1, Issue 2, Pages 113-122 https://doi.org/10.1016/j.jare.2010.03.002
FIALOVÁ, J., CHUMCHALOVÁ, J., MIKOVÁ, K. and HRŮŠOVA, I., 2008. Effect of food preservatives on the growth of spoilage lactobacilli isolated from mayonnaise-based sauces Food Control, 19 , pp. 706-713, 10.1016/j.foodcont.2007.07.018 https://doi.org/10.1016/j.foodcont.2007.07.018
FRENICH, A. G., ROMERO-GONZÁLEZ, R., GÓMEZ-PÉREZ, M. L., and VIDAL, J. L. M., 2011. Multi-mycotoxin analysis in eggs using a QuEChERS-based extraction procedure and ultra-high-pressure liquid chromatography coupled to triple quadrupole mass spectrometry. J. Chromatogr. 1218(28), 4349-4356. https://doi.org/10.1016/j.chroma.2011.05.005
GHAZVINI, R. D., KOUHSARI, E., ZIBAFAR, E., HASHEMI, S. J., AMINI, A., and NIKNEJAD, F., 2016. Antifungal Activity and Aflatoxin Degradation of BifidobacteriumBifidum and Lactobacillus Fermentum Against Toxigenic Aspergillus Parasiticus. Int J Food Microbiol. Open Microbiol J. 30:10:197 http://dx.doi.org/10.2174/1874285801610010197
GRECO M. V., FRANCHI M. L., GOLBA S. L. R., PARDO A. G., and POSE, G. N., 2014. Mycotoxins and mycotoxigenic fungi in poultry feed for food-producing animals. Sci. World J. ;14:1–9. https://doi.org/10.1155/2014/968215
HASHEMI, S. M. B., AMIN, M. K., MOHAMED, K., JAIME, L., SEYED, H. A. Y., SEYEDEH, F. H., MASOUMEH, K., AZAM, M., and SAMIRA, A., 2017. Novel edible oil sources: Microwave heating and chemical properties. Food Research International. volume 92, Pages 147-153. http://dx.doi.org/10.1016/j.foodres.2016.11.033
HASSAN A.M., ABDEL-AZIEM, S.H., EL-NEKEETY, A.A., and ABDEL-WAHHAB, M.A., 2015. Panax ginseng extract modulates oxidative stress, DNA fragmentation and up-regulate gene expression in rats sub chronically treated with aflatoxin B 1 and fumonisin B 1. Cytotechnology. 67, 861-871. https://doi.org/10.1007/s10616-014-9726-z
HERZALLAH, S. M. 2009. Determination of aflatoxins in eggs, milk, meat and meat products using HPLC fluorescent and UV detectors. Food Chemistry 2009; 114(3) 1141-1146. https://doi.org/10.1016/j.foodchem.2008.10.077
IBRAHIM M. H., AMIN, A. R. , TOLBA, S. K., and ELOKLE A. A., 2018. Study on Aflatoxin Residues in some Meat Products and their Control by Probiotics BENHA VETERINARY MEDICAL JOURNAL, VOL. 34, NO. 1:232-241, MARCH, 2018 https://doi.org/10.21608/bvmj.2018.54244
INTERNATIONAL AGENCY FOR RESEARCH ON CANCER (IARC), 2002. Monograph on the evaluation of carcinogenic risk to human. Some traditional herbalmedicines, some mycotoxins, naphthalene and styrene: Summary of data reported and evaluation (Vol. 82)
ISMAEL, S. R., MOHAMMED, B. K., KHIDHIR, Z. K., HUSSEIN, H. M., SHAKER, A. S., and SULAIMANI, K. G. R., 2022. Effect garlic (Allium sativum) powder as an antifungal for wheat crops. Kirkuk University Journal For Agricultural Sciences (KUJAS), 13(4) Pages 456-463 https://doi.org/10.5455%2FOVJ.2022.v12.i5.1
IQBAL, S. Z, NISAR, S., ASI, M. R., and JINAP, S., 2014. Natural incidence of aflatoxins, ochratoxin A and zearalenone in chicken meat and eggs. Food Control. 2014;43:98–103. http://dx.doi.org/10.1016%2Fj.foodcont.2014.02.046
ISO 21527, (2008). Horizontal method for enumeration of yeast and mold in food:part2 colony count techique)
JANG, H.S., JO, H.J., LEE, K.E., and LEE, C., 2007. Survey of the presence of aflatoxins in compound feeds and feed ingredients. Korean J. Fd Hyg. Safety. 2007a;22:346–352. 2465-9223(eISSN)
JANG, H.-S., KIM, D.-H., LEE, K.-E., and LEE C., 2007. Survey of the presence of ochratoxin A in compound feeds and feed ingredients distributed in Korea. Korean J. Fd Hyg. Safety. 2007b;22:353–358. 2465-9223(eISSN)
JIA, R., MA, Q., FAN, Y., JI C., ZHANG, J., LIU T., and ZHAO, L., 2016. The toxic effects of combined aflatoxins and zearalenone in naturally contaminated diets on laying performance, egg quality and mycotoxins residues in eggs of layers and the protective effect of Bacillus subtilis biodegradation product. Food Chem. Toxicol. 90: 142–150. https://doi.org/10.1016/j.fct.2016.02.010
KABAK, B., DOBSON, A. D., and VAR, I., 2006. “Strategies to prevent mycotoxin contamination of food and animal feed: A review,” Critical Reviews in Food Science and Nutrition, vol. 46, no. 8, pp. 593-619,2006. https://doi.org/10.1080/10408390500436185
LEE, N.A., WANG, S., ALLAN, R.D., and KENNEDY, I.R., 2004. A rapid aflatoxin B1 ELISA: Development and validation with reduced matrix effects for peanuts, corn, pistachio and soybeans. J. Agric. Food Chem. 52:2746-2755. https://doi.org/10.1021/jf0354038
MIR, S. A., DAR, B.N., SHAH, M. A., SOFI, S. A., HAMDANI, A.M., CARLOS, A.F., OLIVEIRA , M. H., MOOSAVI, A. M., and KHANEGHAH , A. S., 2021. Application of new technologies in decontamination of mycotoxins in cereal grains: Challenges, and perspectives. Food and Chemical Toxicology (2021) Volume 148, 111976 https://doi.org/10.1016/j.fct.2021.111976
MIRZANAJAFIZANJANI, M., YOUSEFI, M., and EHSANI, A., 2019.Challenges and approaches for production of a healthy and functional mayonnaise sauce. Food Sciences and Nutrition, 7, pp. 2471-2484, 10.1002/fsn3.113.https://doi.org/10.1002%2Ffsn3.1132
MISCHEK, D., and KRAPFENBAUER-CERMAK, C., 2012. Exposure assessment of food preservatives (sulphites, benzoic and sorbic acid) in Austria. FoodAdditives and Contaminants, PartA, 29(3), 371–382. https://doi.org/10.1080/19440049.2011.643415
MORTEZA, A. A., FATEMEH (ELHAM), A., MAHYA, M., and REZA, K. D., 2013. Biological control of aflatoxins. European. J. of Experimental Biology, 3 (2): 162-166. http://www.pelagiaresearchlibrary.com/
MUHIALDIN, B.J., MOHAMMED, N.K.,CHEOK, H.J., FAROUK, A.E.A., and MEOR HUSSIN, A.S., 2021. Reducing microbial contamination risk and improving physical properties of plant-based mayonnaise produced using chickpea aquafaba. International Food Research Journal, 28 (3), pp. 547-553 http://dx.doi.org/10.47836/ifrj.28.3.14
OZDEMIR, N. , BAYRAK, A., TAT, T., NUR YANIK, Z., ALTAY, F., and HALKMAN, A.K., 2021. Fabrication and characterization of basil essential oil microcapsule-enriched mayonnaise and its antimicrobial properties against Escherichia coli and Salmonella Typhimurium. Food Chemistry, 359.https://doi.org/10.1016/j.foodchem.2021.129940
PAIVAA, M.J.M.,SILVAB, M.L., ALCANTARAB, M.R., SANTOSB, F.B.S., COSTAB, J.V.R., DIOGOB, R.F., SILVAA , L.T.F., SANTOSC, A.L., GUEDESD, E.H.S., VELLANOB, P.O., MAGALHAESE, C.C.R.G.N., and DAMASCENOB, I.A.M., 2023. Microbiological evaluation of homemade mayonnaiseand self –serve acai sold in Araguaina, Tocantins.Brazilian journal of biology 83(1) . https://doi.org/10.1590/1519-6984.275603.
Piper, J.D., and Piper, P.W., 2017. Benzoate and sorbate salts: a systemic review of the potential hazards ofthese invaluable preservatives and the expanding spectrum of clinical uses for sodium benzoate. Comprehensive reviews in food science and food safety, 16(5),868-880. https://doi.org/10.1111/1541-4337.12284
Pleadin, J., Frece, J., and Markov, K., (2019. Mycotoxins in food and feed. In Adv. Food Nutr. Res., Elsevier: 89,297-345. https://doi.org/10.1016/bs.afnr.2019.02.007
Qi, N., Yu, H.; Yang, C., Gong, X., Liu, Y., and Zhu, Y., 2019. Aflatoxin B1 in peanut oil from Western Guangdong, China, during 2016–2017. Food Additives & Contaminants: Part B 2019, 12, 45-51, https://doi.org/10.1080/19393210.2018.1544173.
RAY, B., and BHUNIA, A., 2013. Fundamental food microbiology (5th ed.), CRC Press.
RAZAGHI-ABYANEH, M., FAROKH, M. S. G., KAWACHI, M., and ESLAMIFAR, A., 2006. “Ultrastructural evidence of growth inhibitory effect of a novel biocide akacid on an aflatoxigenic aspergillus parasiticus,” Toxicon, vol. 48, pp. 1075-1082. https://doi.org/10.1016/j.toxicon.2006.09.002
ROMINA, P. P., DANTE, J. B., MARIA, R. A., LILIA, C., ANA,M. D., and MARIO, A. S., 2011. Binding of Aflatoxin B1 to Lactic Acid Bacteria and Saccharomyces cerevisiae in vitro: A Useful Model to Determine the Most Efficient Microorganism, Aflatoxins - Biochemistry and Molecular Biology, ISBN: 978-953-307-395-8, In Tech, 323:346. http://dx.doi.org/10.5772/23717
SADIQ, F. A., YAN, B., TIAN, F., ZHAO, J., ZHANG, H., and CHEN, W., 2019. Lactic acid bacteria as antifungal and anti‐mycotoxigenic agents: a comprehensive review. Comprehensive Reviews in Food Science and Food Safety, 18(5), 1403-1436. https://doi.org/10.1111/1541-4337.12481
SHEHATA, A. A., ALI, H., and GHAZALI, N. H., 2014. Detection of Aflatoxin and antibacterial residues in different types of table eggs with studying of the effect of heat treatment. Benha Veter Med J. 27(2), 177-187. http://www.bvmj.bu.edu.eg
Silvia, G. 2007: Aflatoxin binding by probiotics: Experimental studies on intestinal aflatoxin transport, metabolism and toxicity. Kuopio University Publications D. Medical Sciences 404, 85 p.
TENEVA, D.,DENKOVA, Z., DENKOVA,Z., KOSTOVA, B., GORANOV, G., KOSTOV, A., SLAVCHEV, Y., HRISTOVA-IVANOVA, G., and UZUNOVA, D. P., 2021. Biological preservation of mayonnaise with Lactobacillus plantarum LBRZ12, dill, and basil essential oils, Food Chemistry, 344 (2021), https://doi.org/10.1016/j.foodchem.2020.128707
THIRUMALA-DEVI K., MAYO M. A., REDDY G., and REDDY D. V. R. 2002. Occurrence of aflatoxin and ochratoxin A in Indian poultry feeds. J. Food Prot. 2002; 65: 1338–1340. http://dx.doi.org/10.4315/0362-028X-65.8.1338
YALING, W., TONGJIE, C., GUOZHONG, L., CHUNSAN, Q., HUIYONG, D., MEILING, Y., and GERD, S., 2008. Similtaneous detection of airborne aflatoxin, ochratoxin and zearalenone in poultry houses by immunoaffinity column and high-performance liquid chromatography. Environ. Res 2008;107, 139-144. https://doi.org/10.1016/j.envres.2008.01.008
YOLMEH, M., HABIBINAJAFI, M.B., FARHOOSH, R., and SALEHI, F., 2014. Modeling of antibacterial activity of annatto dye on Escherichia coli in mayonnaise, Food Bioscience, 8 , pp. 8-13, https://doi.org/10.1016/J.FBIO.2014.09.001
ZAIN, M.E. 2011. Impact of mycotoxins on humans and animals. J. Saudi Chem. Soc. 15,129–144. https://doi.org/10.1016/j.jscs.2010.06.006 | ||||
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