DEVELOPMENT OF AN IMMUNOPEROAIDASE TEST FOR TURKEY RHINOTRACHITIS SERODIAGNOSIS
M, S., KOCH, G. (1995). DEVELOPMENT OF AN IMMUNOPEROAIDASE TEST FOR TURKEY RHINOTRACHITIS SERODIAGNOSIS. EKB Journal Management System, 43(2), 193-200. doi: 10.21608/vmjg.1995.375685
SHAKAL M; G KOCH. "DEVELOPMENT OF AN IMMUNOPEROAIDASE TEST FOR TURKEY RHINOTRACHITIS SERODIAGNOSIS". EKB Journal Management System, 43, 2, 1995, 193-200. doi: 10.21608/vmjg.1995.375685
M, S., KOCH, G. (1995). 'DEVELOPMENT OF AN IMMUNOPEROAIDASE TEST FOR TURKEY RHINOTRACHITIS SERODIAGNOSIS', EKB Journal Management System, 43(2), pp. 193-200. doi: 10.21608/vmjg.1995.375685
M, S., KOCH, G. DEVELOPMENT OF AN IMMUNOPEROAIDASE TEST FOR TURKEY RHINOTRACHITIS SERODIAGNOSIS. EKB Journal Management System, 1995; 43(2): 193-200. doi: 10.21608/vmjg.1995.375685

DEVELOPMENT OF AN IMMUNOPEROAIDASE TEST FOR TURKEY RHINOTRACHITIS SERODIAGNOSIS

Veterinary Medical Journal (Giza)
Volume 43, Issue 2, April 1995, Page 193-200  XML
Document Type: Original Article
DOI: 10.21608/vmjg.1995.375685
View on SCiNiTO View on SCiNiTO
Authors
SHAKAL M* 1; G KOCH2
1Department of Poultry Dis., Fac. Vet. Med. Med., Cairo Univ..
2Central Veterinary Institute, The Netherlands, Leylstad.
Abstract
The development of an indirect immunoperoxidase test for turkey rhinotracheitis (TRT) serodiagnosis is described utilising CER-monolayers. There was no nonspecific binding of Newcastle or influenza antibodies to TRT virus infected plates. Field sera from vaccinated and nonvaccinated turkeys of different ages have been tested. Immunoperoxidase test showed higher sensitivity than serum neutralisation test. The specific bar-like cytoplasmic staining has been demonstrated with positive sera proving the specificity of staining. Nonspecific peroxidase background staining was excluded through preincubation of tested sera on noninfected monolayers. Glycerol preserved plates could be kept as long as 6 months at 4-8°C, and shortened the test time to 2-3 hours.
Statistics
Article View: 44