Genetic Diversity of GPAM, DGAT1, and SCD1 Lipogenic Candidate Genes In Egyptian and Indian Buffalo | ||
| Egyptian Journal of Veterinary Sciences | ||
| Article 18, Volume 56, Issue 11, November 2025, Pages 2895-2908 PDF (1.24 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2024.302279.2234 | ||
| Authors | ||
| Sonia Sihag1; Mohamed A Zahran* 2; Hamdy Abdel-Shafy2; Vinod Chhokar1; Anil Kumar1; samy Abou-Bakr2; Mohamed s Hassanane3; Hossam E. Rushdi2 | ||
| 1Department of Bio & Nano Technology, Guru Jambheshwar University of Science & Technology, Hisar-125001, Haryana, India. | ||
| 2Department of Animal Production, Faculty of Agriculture, Cairo University, Algammaa Street, 12613 Giza, Egypt. | ||
| 3Cell Biology Department, National Research Center, 12622 Dokki, Giza, Egypt | ||
| Abstract | ||
| The objectives of the present study were to analyze polymorphisms in the glycerol-3-phosphate acyltransferase mitochondrial (GPAM), diacylglycerol O-acyltransferase 1 (DGAT1), and stearoyl-coenzyme A desaturase 1 (SCD1) genes in the Egyptian buffalo breed, and also to explore genetic diversity across Egyptian, Murrah, and Bhadawari buffalo populations. Genotyping was conducted using PCR-SSCP and sequencing techniques. Results indicate significant deviations from Hardy–Weinberg equilibrium (HWE) in the Egyptian buffalo breed for GPAM, DGAT1, and SCD1 genes, with Murrah significantly differing at DGAT1 and SCD1 genes. Bhadawari breed significantly deviated from HWE only at DGAT1 gene. Pairwise comparison between populations revealed the shortest genetic distance between Egyptian and Murrah, while the longest between Bhadawari and Murrah. Multiple SNPs were identified in the genes under study, with alterations of amino acid sequences. These findings reveal novel genetic variants with potential implications for lipid metabolism and milk quality in Egyptian buffalo. Further research is warranted to elucidate the functional significance and breeding applications of these variations. | ||
| Keywords | ||
| River buffalo; GPAM; DGAT1; SCD1; PCR-SSCP; SNPs; Genotyping | ||
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