EVALUAT ION OF A RECOMBINANT ELISA ANTIGEN FOR SERODIAGNOSIS OF BOVINE VIRAL DIARRHEA IN IMMUNE AND INFECTED CATTLE SERA IN EGYPT | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 48, Issue 2, April 2000, Page 227-238 PDF (4.13 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2000.376270 | ||||
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| Authors | ||||
| A EL-KHOLY; H GHALY; SAMIRA SAID; M EL-SABBAGH | ||||
| Veterinary Serum & Vaccine Research Institute, ARC, P.O.Box 131, Abassia, Cairo, Egypt. | ||||
| Abstract | ||||
| The recombinant protein (r1448) in crude lysate of insect cells infected with a previously devcl-oped recombinant baculovirus, expressing two structural protcins, the nucleocapsid protein (p14) and the envelope glycoprotein (gp48) of the NADL strain of bovine viral diarrhea virus (BVDV), was evaluated as a coating antigen in a Standard enzyme-linked immunosorbent assay (ELISA) for detection of antibody against homol- ogous and heterologous strains of BVDV. The ELISA was compared to standard virus neutrali-zation test (VNT) for detection of antibody against the local Iman strain of BVDV in sera of calves immunized with the locally produced Pneumo-3 inactivated vaccine, experimentally in- fected calves and contact controls. Antibody titers With ELISA were closcly corrclatcd ‘ibody titers estimated by VNT (averager= 0.917). Nevertheless, thc recombinant protcin (r1448) was an effective and inexpensive ELISA antigen for detection of antibody against homolo- gous and heterologous strains of both gcnotypcs of BVDV (BVDVI and BVDV2) In reference hyperimmune cattle scra. In conclusion, the recombinant antigen (r1448) is a reliable candi-date for diagnostic tests of BVDV infections in Egypt. | ||||
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