EVULATION OF INDIRECT ELISA TEST In DIAGNOSIS OF BRUCELLOSIS IN CAMELS | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 48, Issue 4, October 2000, Page 467-477 PDF (3.51 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2000.376549 | ||||
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| Author | ||||
| M HAMDY | ||||
| Department of Brucellosis, Animal Health Research Institute Dokki, Giza, Egypt | ||||
| Abstract | ||||
| A serological survey was carried out on 365 camels (Camelus dromedaries, one humped camel) in Saudi Arabia. Out of these 250 were Imported from the Sudan, 80 from the Norther Abattoir and 35 blood samples were collected om local markets in Jeddah, Saudi Arabia. Examintion Of these samples with Standard tube wn (SAT), Rose Bengal plate test hay «nt acidified plate antigen test and rivanol test (Riv. T) was done. and their Positive result were (6.85%), (9.59%), (12.02%) and (7.67%) respectively. application of the indirect ELISA on the same camel sera using antibovine, anti ovine amd antigoat conjugate revealed positive results of (11.5 %), (7.4%) and (6.8) respectively. the technique of ELISA with anti bovine conjugate showed excellent agreement with BAPAT, as a sensitive test with Kappa values of 0.947 and good agreement with Riv .t. as a specific test, with K values of0.78. The ELISA with anti bovine conjugate was proved to be sensetive as well as specific test for examining camels for brucellosis. anti bovine conjugate in ELISA correlated very well with camel sera than the antiovine or antigoat conjugate. two brucella isoletes were obtained from five aborted foeti. while no brucella isolates were obtained from 35 camel milk samples. the isolated strains proved to be brucella melitensis biovar3. | ||||
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