PREPRATION OF A SPECIFIC INACTIVATED PESTE DES PETITS RUMINATS VACCINE | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 48, Issue 4, October 2000, Pages 625-632 PDF (2.37 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2000.376590 | ||
| Authors | ||
| A FAYED1; M KHODEIR2; M MOUAZ2; NAHED KAMEL1 | ||
| 1Infectious diseases department, Veterinary medicine, Cairo University. | ||
| 2Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo. | ||
| Abstract | ||
| Cell culture adapted local strain of peste des petit ruminants virus "PPRV" (Egypt-87), was inacti-vated by treatment with binary ethyleneimine(BEI) at the concentrations of 1%, 2% and 3%. Aconcentration of 3% of 0.1 M BEI was found to be the faster one to obtain complete inactivation of 106.5 TCIDSO PPRV within 2 hours. 20% al-iydragel was added as an adjuvant and different doses Were tested to immunize sheep. There was 0 significant differences in the immune responseof animals vaccinated with either 1, 2 or 3ml of the inactivated vaccine. Boostering dose after 1 baton , ne resulted in a better duration of immunity extending to 8 months while it was 5 months on using a single dose of the vaccine . | ||
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