USE OF WESTERN BLOT TECHNIQUE FOR THE DETECTION OF THE IMMUNOGENIC DETERMINTS OF CLOSTRIDIUM CHAUVOEL WHOLE CELLULAR ANTIGEN
M.EL-BARDISY, M., GABAL, M. (1998). USE OF WESTERN BLOT TECHNIQUE FOR THE DETECTION OF THE IMMUNOGENIC DETERMINTS OF CLOSTRIDIUM CHAUVOEL WHOLE CELLULAR ANTIGEN. EKB Journal Management System, 46(2), 171-176. doi: 10.21608/vmjg.1998.377265
M. M.EL-BARDISY; M.A GABAL. "USE OF WESTERN BLOT TECHNIQUE FOR THE DETECTION OF THE IMMUNOGENIC DETERMINTS OF CLOSTRIDIUM CHAUVOEL WHOLE CELLULAR ANTIGEN". EKB Journal Management System, 46, 2, 1998, 171-176. doi: 10.21608/vmjg.1998.377265
M.EL-BARDISY, M., GABAL, M. (1998). 'USE OF WESTERN BLOT TECHNIQUE FOR THE DETECTION OF THE IMMUNOGENIC DETERMINTS OF CLOSTRIDIUM CHAUVOEL WHOLE CELLULAR ANTIGEN', EKB Journal Management System, 46(2), pp. 171-176. doi: 10.21608/vmjg.1998.377265
M.EL-BARDISY, M., GABAL, M. USE OF WESTERN BLOT TECHNIQUE FOR THE DETECTION OF THE IMMUNOGENIC DETERMINTS OF CLOSTRIDIUM CHAUVOEL WHOLE CELLULAR ANTIGEN. EKB Journal Management System, 1998; 46(2): 171-176. doi: 10.21608/vmjg.1998.377265

USE OF WESTERN BLOT TECHNIQUE FOR THE DETECTION OF THE IMMUNOGENIC DETERMINTS OF CLOSTRIDIUM CHAUVOEL WHOLE CELLULAR ANTIGEN

Veterinary Medical Journal (Giza)
Volume 46, Issue 2, April 1998, Page 171-176  XML PDF (2.54 MB)
DOI: 10.21608/vmjg.1998.377265
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Authors
M. M.EL-BARDISY* 1; M.A GABAL2
1Animal Health Rescarch Institute, Dokki, Egypt.
2Dept. of Microbiology, Immunology and Preventive Medicine, ISU.
Abstract
Sodium dodcyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used for fractionation of the whole cellular antigens of C.chauvoei. The obtained results revealed the presence of 8 polypeptide bands which ranged from 205 to 18.5 KDa. A major protein band with a molecular weight of 49.5 KDa was identified. Application of the immunoblotting technique with the specific hyperimmune serum prepared against C.chauvoei whole cellular formalinized antigen in rabbits, showed the presence of some major antigenic epitopes which reacted strongly with the specific hyperimmune serum, mainly at 29.5 and 205 KDa.
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