DETECTION OF TOXIGENIC PASTEURELLA MULTOCIDA BY THE POLYMERASE CHAIN REACTION (PCR) IN FIFLD SAMPLES
A.S, A., N.E., E., M A.I., T., E.R.J., A., A.I., S. (1998). DETECTION OF TOXIGENIC PASTEURELLA MULTOCIDA BY THE POLYMERASE CHAIN REACTION (PCR) IN FIFLD SAMPLES. EKB Journal Management System, 46(4), 443-449. doi: 10.21608/vmjg.1998.377324
AMIN A.S; EL-BATRAWY N.E.; TANIOS M A.I.; ATTIA E.R.J.; SHAABAN A.I.. "DETECTION OF TOXIGENIC PASTEURELLA MULTOCIDA BY THE POLYMERASE CHAIN REACTION (PCR) IN FIFLD SAMPLES". EKB Journal Management System, 46, 4, 1998, 443-449. doi: 10.21608/vmjg.1998.377324
A.S, A., N.E., E., M A.I., T., E.R.J., A., A.I., S. (1998). 'DETECTION OF TOXIGENIC PASTEURELLA MULTOCIDA BY THE POLYMERASE CHAIN REACTION (PCR) IN FIFLD SAMPLES', EKB Journal Management System, 46(4), pp. 443-449. doi: 10.21608/vmjg.1998.377324
A.S, A., N.E., E., M A.I., T., E.R.J., A., A.I., S. DETECTION OF TOXIGENIC PASTEURELLA MULTOCIDA BY THE POLYMERASE CHAIN REACTION (PCR) IN FIFLD SAMPLES. EKB Journal Management System, 1998; 46(4): 443-449. doi: 10.21608/vmjg.1998.377324

DETECTION OF TOXIGENIC PASTEURELLA MULTOCIDA BY THE POLYMERASE CHAIN REACTION (PCR) IN FIFLD SAMPLES

Veterinary Medical Journal (Giza)
Volume 46, Issue 4, October 1998, Page 443-449  XML PDF (2.08 MB)
DOI: 10.21608/vmjg.1998.377324
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Authors
AMIN A.S* 1; EL-BATRAWY N.E.2; TANIOS M A.I.3; ATTIA E.R.J.2; SHAABAN A.I.3
1Animal Reproduction Research Institute, Giza, Egypt
2Animal Reproduction Research Institute, Giza, Egypt.
3Animal Health Research Institute, Giza, Egypt.
Abstract
Pasteurella multocida is an important cause of pneumonia in newly born animals, usually as a secondary pathogen invading lungs injured by other bacteria OF viruses. Toxigenic and notoxigenic Pasteurella multocida isolates can not be differentiated by morphology oF standard biochemical reaction. A more rapid and accurate method to detect toxigenic Pasteurella multocida is needed for improving clinical diagnosis, prevention , treatment and epidemiological studies. the feasibility of using polymerase chain reaction (PCR) amplification assay for accurate and rapid detection of toxigenic Pasteurella multocida in field samples was studied. The results showed that PCR assay could detect toxigenic Pasteurella Multocida antigen in field samples. The result Indicate that there was complete agreement of the Toxigenic status of the Pasteurella multocida Based on mouse lethality and PCR assay.the Concordance of PCR results with the defined toxigenic status indicates 100% specificity and sensitivity.The PCR protocol could be completed within one working day which is faster and more sensitive than the time consuming conventional diagnostic techniques currently used in clinical laboratories.
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