DETECTION OF BOVINE HERPES NIRUS TYPE 1 IN SEMEN USING A RAPID AND SENSITIVE POLYMERASE CHAIN REAACTION ASSAY
A.S., A., A.K., I. (1998). DETECTION OF BOVINE HERPES NIRUS TYPE 1 IN SEMEN USING A RAPID AND SENSITIVE POLYMERASE CHAIN REAACTION ASSAY. EKB Journal Management System, 46(4), 463-469. doi: 10.21608/vmjg.1998.377330
AMIN A.S.; IBRAHIM A.K.. "DETECTION OF BOVINE HERPES NIRUS TYPE 1 IN SEMEN USING A RAPID AND SENSITIVE POLYMERASE CHAIN REAACTION ASSAY". EKB Journal Management System, 46, 4, 1998, 463-469. doi: 10.21608/vmjg.1998.377330
A.S., A., A.K., I. (1998). 'DETECTION OF BOVINE HERPES NIRUS TYPE 1 IN SEMEN USING A RAPID AND SENSITIVE POLYMERASE CHAIN REAACTION ASSAY', EKB Journal Management System, 46(4), pp. 463-469. doi: 10.21608/vmjg.1998.377330
A.S., A., A.K., I. DETECTION OF BOVINE HERPES NIRUS TYPE 1 IN SEMEN USING A RAPID AND SENSITIVE POLYMERASE CHAIN REAACTION ASSAY. EKB Journal Management System, 1998; 46(4): 463-469. doi: 10.21608/vmjg.1998.377330

DETECTION OF BOVINE HERPES NIRUS TYPE 1 IN SEMEN USING A RAPID AND SENSITIVE POLYMERASE CHAIN REAACTION ASSAY

Veterinary Medical Journal (Giza)
Volume 46, Issue 4, October 1998, Pages 463-469    PDF (2.29 M)
DOI: 10.21608/vmjg.1998.377330
Authors
AMIN A.S.* 1; IBRAHIM A.K.2
1Animal Reproduction Research Institute, Giza, Egypt.
2Fac. Vet. Med.,Cairo Univ.
Abstract
polymerase Chain Reaction (PCR) for the detection of bovine herpesvirus type1 | (BHV-1) was evaluated. Since bovine semen contains components that inhibit PCR amplification, a protocol was used to purify BHV-1 DNA from semen. The study to compare the PCR assay with the routinely used virus isolation method showed that the PCR assay is 100-fold more sensitive. In addition, the results of the PCR assay are available in 1 day, whereas the virus isolation method takes 10-17 days. It is concluded that the PCR assay is the most sensitive technique for laboratory detection of BHV-I.
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