Elevated CDR1as may affect CACNG5 and EGFR via hsa-miR-7-5p sponge in childhood dilated cardiomyopathy patients.
Esmaiel, N., LA., I., Fayez, A., Ammar, T., El-Bassyouni, H. (2023). Elevated CDR1as may affect CACNG5 and EGFR via hsa-miR-7-5p sponge in childhood dilated cardiomyopathy patients.. EKB Journal Management System, 12(2), 1-9. doi: 10.21608/MXE.2024.377823
Nora N. Esmaiel; Ibrahim LA.; Alaaeldin Fayez; Tamer H.A. Ammar; Hala T. El-Bassyouni. "Elevated CDR1as may affect CACNG5 and EGFR via hsa-miR-7-5p sponge in childhood dilated cardiomyopathy patients.". EKB Journal Management System, 12, 2, 2023, 1-9. doi: 10.21608/MXE.2024.377823
Esmaiel, N., LA., I., Fayez, A., Ammar, T., El-Bassyouni, H. (2023). 'Elevated CDR1as may affect CACNG5 and EGFR via hsa-miR-7-5p sponge in childhood dilated cardiomyopathy patients.', EKB Journal Management System, 12(2), pp. 1-9. doi: 10.21608/MXE.2024.377823
Esmaiel, N., LA., I., Fayez, A., Ammar, T., El-Bassyouni, H. Elevated CDR1as may affect CACNG5 and EGFR via hsa-miR-7-5p sponge in childhood dilated cardiomyopathy patients.. EKB Journal Management System, 2023; 12(2): 1-9. doi: 10.21608/MXE.2024.377823

Elevated CDR1as may affect CACNG5 and EGFR via hsa-miR-7-5p sponge in childhood dilated cardiomyopathy patients.

Middle East Journal of Medical Genetics
Volume 12, Issue 2, July 2023, Pages 1-9    PDF (463.34 K)
Document Type: Original Article
DOI: 10.21608/MXE.2024.377823
Authors
Nora N. Esmaiel* 1; Ibrahim LA.2; Alaaeldin Fayez3; Tamer H.A. Ammar4; Hala T. El-Bassyouni5
1Molecular Genetics and Enzymology Department, Human Genetics and Genome Research Institute, National Research Centre, Giza, Egypt.
2Faculty of Medicine, Cairo University, Cairo, Egypt.
3Molecular Genetics and Enzymology Department, Human Genetics and Genome Research Institute, National Research Centre, Giza, EGYPT.
4Medical Molecular Genetics Department, Human Genetics and Genome Research Institute, National Research Centre, Giza, EGYPT.
5Clinical Genetics Department, Human Genetics and Genome Research Institute, National Research Centre, Giza, EGYPT.
Abstract
Background: In the present study, we mainly aimed to evaluate CDR1as and hsa-circRNA_105039 biomarkers expression in childhood dilated cardiomyopathy (DCM) and ventricular septal defects (VSD) patients. Circular RNAs (circRNAs) are non-coding RNAs that result from the back splicing of pre-mRNA. Circular RNA's exhibit enhanced stability with numerous biological functions. Thus, this circRNAs are promising targets for developing diagnostic tools and therapies for the human diseases. Methods: Fold change of CDR1as and hsa-circRNA_105039 was detected by qRT-PCR in 101 participants. The diagnostic accuracy of CDR1as was determined using receiver operating characteristic curve analysis. To predict CDR1as/miRNAs and CDR1as/proteins interaction networks related to DCM and VSD pathogenesis, gene ontology (GO) and KEGG pathway analyses were performed. Results: CDR1as showed significant higher fold change (FC= 2.9) in DCM group than both control and VSD groups. Experimental evidence-based GO and KEGG pathways analyses showed that CDR1as has 73 miRNAs binding sites on hsa-miR-7-5p which targets 3'UTR of mRNAs involved in MAPK signaling pathway. Conclusion: The potential molecular mechanistic effect of the elevated CDR1as could be concluded by sponging of hsa-miR-7-5p in childhood DCM patients. Consequently, further decreasing of hsa-miR-7-5p may lead to increased dosage of CACNG5 and EGFR which involved in the MAPK signaling pathway.
Keywords
CDR1as; Circular RNAs; Dilated cardiomyopathy; hsa-circRNA_105039; MAPK signaling pathway
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