Histological Study on the Possible Protective Effect of Pumpkin Seed Oil on Cyclophosphamide Induced Hemorrhagic Cystitis in Adult Male Albino Rat
ElHeety, A., Elshwaihy, E., El-deeb, T., Elkelany, M. (2024). Histological Study on the Possible Protective Effect of Pumpkin Seed Oil on Cyclophosphamide Induced Hemorrhagic Cystitis in Adult Male Albino Rat. EKB Journal Management System, 2(2), 157-194. doi: 10.21608/ejctr.2024.283521.1015
Asmaa Mohamed ElHeety; Esam Mohammed Elshwaihy; Thoraya Abd El-Aziz El-deeb; Maram Mohamed Elkelany. "Histological Study on the Possible Protective Effect of Pumpkin Seed Oil on Cyclophosphamide Induced Hemorrhagic Cystitis in Adult Male Albino Rat". EKB Journal Management System, 2, 2, 2024, 157-194. doi: 10.21608/ejctr.2024.283521.1015
ElHeety, A., Elshwaihy, E., El-deeb, T., Elkelany, M. (2024). 'Histological Study on the Possible Protective Effect of Pumpkin Seed Oil on Cyclophosphamide Induced Hemorrhagic Cystitis in Adult Male Albino Rat', EKB Journal Management System, 2(2), pp. 157-194. doi: 10.21608/ejctr.2024.283521.1015
ElHeety, A., Elshwaihy, E., El-deeb, T., Elkelany, M. Histological Study on the Possible Protective Effect of Pumpkin Seed Oil on Cyclophosphamide Induced Hemorrhagic Cystitis in Adult Male Albino Rat. EKB Journal Management System, 2024; 2(2): 157-194. doi: 10.21608/ejctr.2024.283521.1015

Histological Study on the Possible Protective Effect of Pumpkin Seed Oil on Cyclophosphamide Induced Hemorrhagic Cystitis in Adult Male Albino Rat

Egyptian Journal of Cell and Tissue Research
Volume 2, Issue 2, June 2024, Pages 157-194    PDF (4.84 M)
Document Type: Original articles
DOI: 10.21608/ejctr.2024.283521.1015
Authors
Asmaa Mohamed ElHeety1; Esam Mohammed Elshwaihy2; Thoraya Abd El-Aziz El-deeb3; Maram Mohamed Elkelany* 4
1Demonstrator of Histology and Cell Biology Department, Faculty of Medicine, Tanta University, Tanta, 31527, Egypt.
2Assistant Professor of Histology and Cell Biology Department, Faculty of Medicine, Tanta University, Tanta, 31527, Egypt.
3Professor of Histology and Cell Biology Department, Faculty of Medicine, Tanta University, Tanta, 31527, Egypt.
4Lecturer of Histology and Cell Biology Department, Faculty of Medicine, Tanta University, Tanta, 31527, Egypt.
Abstract
Background: Cyclophosphamide (CYP) is an alkylating drug used in cancer treatment, one of its common complications is hemorrhagic cystitis (HC). Pumpkin seed oil (PSO) is a strong anti-inflammatory, antioxidant and anticancer. Aim of the Work: This study was carried out to study the possible protective effect of pumpkin seed oil on cyclophosphamide induced hemorrhagic cystitis. Materials & Methods: The present study was carried out on 45 adult male albino rats. They were randomly divided into four main groups. Group I represented the control group. Group ll (PSO) received PSO at a dose of (40mg/kg/day) orally for 28 days. Group lll (HC) received CYP solution (75 mg/kg) for induction of HC. Group IV (PSO+HC) received PSO for 28 days as in group II, and at the 20th day, HC was induced by the same dose and manner as in group III. The urinary bladder specimens were processed for different histological techniques. Morphometrical and statistical studies were also performed. Results: Group III showed a lot of changes at the level of light microscopic study including epithelial ulceration, nuclear changes, hemorrhage, extensive fibrosis in Masson Trichrome stained sections and weak PAS reaction. Furthermore, HC group showed strong positive immune reaction in the Bax immunostained sections. These findings were confirmed in our morphometric data. Additionally, ultrastructural examination of HC group showed rarefied cytoplasm, widening of intercellular spaces and swollen mitochondria. Meanwhile, group IV showed marked improvement of the previously mentioned pathological changes. Conclusion: PSO administration plays a protective role in cyclophosphamide-induced hemorrhagic cystitis.
Keywords
Cyclophosphamide; PSO; urinary bladder; Bax; ultrastructure
References
 

  1. Mbanefo, E. C., Le, L., Zee, R., Banskota, N., Ishida, K., Pennington, L. F., Odegaard, J. I., Jardetzky, T. S., Alouffi, A., & Falcone, F. H. (2019). IPSE, a urogenital parasite-derived immunomodulatory protein, ameliorates ifosfamide-induced hemorrhagic cystitis through downregulation of pro-inflammatory pathways. Scientific Reports, 9(1), 1586,1-14.
  2. Swan, D., Gurney, M., Krawczyk, J., Ryan, A. E., & O’Dwyer, M. (2020). Beyond DNA damage: exploring the immunomodulatory effects of cyclophosphamide in multiple myeloma. Hemasphere, 4(2), e350,1-10.
  3. Zhang, Z., Pan, T., Liu, C., Shan, X., Xu, Z., Hong, H., Lin, H., Chen, J., & Sun, H. (2021). Cyclophosphamide induced physiological and biochemical changes in mice with an emphasis on sensitivity analysis. Ecotoxicology and Environmental Safety, 211, 111889,1-6.
  4. Elmeligy, M. H., Farid, A. S., and Fararh, K. (2019): Antioxidant and hepatoprotective effect of pumpkin seed oil in CCl4-intoxicated rats. Benha Veterinary Medical Journal, 36(2): 77-89.
  5. Hamdi, H., and Hassan, M. M. (2021): Maternal and developmental toxicity induced by nanoalumina administration in albino rats and the potential preventive role of the pumpkin seed oil. Saudi Journal of Biological Sciences, 28(8): 4778-4785.‏
  6. Dobrek, L., Nalik-Iwaniak, K., Fic, K., & Arent, Z. (2020). The effect of acetylcysteine on renal function in experimental models of cyclophosphamide-And ifosfamide-induced cystitis. Current Urology, 14(3), 150–162.
  7. El-Azma, M. H., El-Beih, N. M., El-Shamy, K. A., Koriem, K. M. M., Elkassaby, M. I., & El-Sayed, W. M. (2022). Pumpkin seed oil and zinc attenuate chronic mild stress perturbations in the cerebral cortex of rats. Nutrition & Food Science, 52(7), 1070–1082.
  8. Ghasi, S. I., Umana, I. K., Ogbonna, A. O., Nwokike, M. O., & Ufelle, S. (2020). Cardioprotective effects of animal grade piperazine citrate on isoproterenol induced myocardial infarction in wistar rats: Biochemical and histopathological evaluation. African Journal of Pharmacy and Pharmacology, 14(8), 285–293.
  9. Suvarna, K.S., Layton, C. and Bancroft, J.D. (2013): Bancroft’s Theory and Practice of Histological Techniques. 7th edition, Elsevier, Churchill Livingstone, chapters 6-12, pp:105-224.
  10. Kassab, A. A. (2018). Wheat germ oil attenuates deltamethrin-induced injury in rat cerebellar cortex: Histological and immunohistochemical study. Egyptian Journal of Histology, 41(2), 182–191.
  11. Magaki, S., Hojat, S. A., Wei, B., So, A., & Yong, W. H. (2019). An introduction to the performance of immunohistochemistry. Biobanking: Methods and Protocols, 289–298.
  12. Elabd, S. S., Ibrahim, M. A., & Sadek, M. T. (2022). Effect of Tadalafil on Apoptosis and Proliferation in Urinary Bladder Mucosa after Chemically Induced Hemorrhagic Cystitis; A Histological and Immunohistochemical Study. Egyptian Journal of Histology, 45(1), 1–16.
  13. Suvarna, K.S., Layton, C. and Bancroft, J.D. (2019): Bancroft's Theory and Practice of Histological Techniques (Eighth ed.): Elsevier Health Sciences.China, chapter 21, Transmission electron microscopy. P:434-440.
  14. El Shaer, D. F., & Elkelany, M. M. (2023). Adverse Effect of Dexamethasone on the Thyroid Gland of Adult Male Albino Rat and the Possible Protective Role of Curcumin: Histological, Immunohistochemical and Biochemical Study. Egyptian Journal of Histology, 46(2), 619–634.
  15. Mahmoud, M. S. (2020). Gastroprotective activity of olive leaves extract on 2, 3, 7, 8 tetrachlorodibenzo-p-dioxin induced gastric fundic mucosal injury in adult male albino rats (light and electron microscopic study). Egyptian Journal of Histology, 43(4), 1188–1204.
  16. Davis, H.T. and Crombie, L.K (2009): What are confidence intervals and p-values?. 2nd edition, Hayward Group Ltd, London, United Kingdom. P: 1-6.
  17. Ibrahim, K. M., Darwish, S. F., Mantawy, E. M., & El-Demerdash, E. (2023). Molecular mechanisms underlying cyclophosphamide-induced cognitive impairment and strategies for neuroprotection in preclinical models. Molecular and Cellular Biochemistry, 1–21.
  18. Haiba, D. A., & Ibrahim, M. A. A. (2015). Role of dexamethasone alone and in combination with quercetin in the urinary bladder after administration of ifosfamide: a histological, immunohistochemical, and morphometric study. Egyptian Journal of Histology, 38(2), 196–209.
  19. Aboulhoda, B. E., Amin, S. N., Thomann, C., Youakim, M., & Hassan, S. S. (2020). Effect of thymoquinone on cyclophosphamide-induced injury in the rat urinary bladder. Archives of Medical Science, 16(1),1-12. DOI:5114/aoms.2020.97061.
  20. Elsisy, R. A., Taha, M. F., Sonpol, H. M. A., Baokbah, T. A. S., Abdelkareem, M. A., & Farage, A. E. (2021). Acute effect of cyclophosphamide on rat’s urinary bladder and the possible protective role of sulforaphane: a histological and ultrastructural study. International Journal of Scientific Reports, 7(4), 207-214.
  21. Essawy, A. S., Issa, N. M., & Tayel, S. G. (2022). Glycyrrhiza glabra root extract alleviates cyclophosphamide induced mucositis of the tongue in adult male albino rats. Egyptian Journal of Histology, 45(4), 1222–1234.
  22. Rooney, P., Srivastava, A., Watson, L., Quinlan, L. R., & Pandit, A. (2015). Hyaluronic acid decreases IL-6 and IL-8 secretion and permeability in an inflammatory model of interstitial cystitis. Acta Biomaterialia, 19, 66–75.
  23. Abdelmoez, W. A. (2022). Evaluation of the Protective Role of Intravesical Chondroitin Sulfate Versus Intravesical Dimethyl Sulfoxide on The Glycosaminoglycan’s, Uroplakin and Restoration of Barrier Function of the Urinary Bladder in A Rat model of Ketamine Induced Interstitial C. Egyptian Academic Journal of Biological Sciences, D. Histology & Histochemistry, 14(2), 201–214.
  24. Awadallah, N., Proctor, K., Joseph, K. B., Delay, E. R., & Delay, R. J. (2020). Cyclophosphamide has long-term effects on proliferation in olfactory epithelia. Chemical Senses, 45(2), 97–109.
  25. Zupančič, D., Vidmar, G., & Jezernik, K. (2009). Melatonin prevents the development of hyperplastic urothelium induced by repeated doses of cyclophosphamide. Virchows Archiv, 454, 657–666.
  26. Chou, W. H., McGregor, B., Schmidt, A., Carvalho, F. L. F., Hirsch, M. S., Chang, S. L., Kibel, A., & Mossanen, M. (2021). Cyclophosphamide-associated bladder cancers and considerations for survivorship care: A systematic review. Urologic Oncology: Seminars and Original Investigations, 39(10), 678–685.
  27. Chaitanya, G. V., Eeka, P., Munker, R., Alexander, J. S., & Babu, P. P. (2011). Role of cytotoxic protease granzyme‐b in neuronal degeneration during human stroke. Brain Pathology, 21(1), 16–30.
  28. Batista, C., Mota, J., Souza, M. L. P., Leitão, B. T. A., Souza, M., Brito, G. A. C., Cunha, F. de Q., & Ribeiro, R. A. (2007). Amifostine and glutathione prevent ifosfamide-and acrolein-induced hemorrhagic cystitis. Cancer Chemotherapy and Pharmacology, 59, 71–77.
  29. Khorwal, G., Chauhan, R., Nagar, M., & Khorwal, G. (2017). Effect of cyclophosphamide on liver in albino rats: A comparative dose dependent histomorphological study. International Journal of Biomedical and Advance Research, 8(3), 102–107.
  30. Bashandy, M. A., & Zedan, O. I. (2019). Role of alpha lipoic acid on cyclophosphamide induced cardiotoxicity in adult male albino rat: Histological study. Egyptian Journal of Histology, 42(4), 888–899.
  31. Attia, A. A., Sorour, J. M., Mohamed, N. A., Mansour, T. T., AlEisa, R. A., & El-Shenawy, N. S. (2023). Biochemical, Histological, and Ultrastructural Studies of the Protective Role of Vitamin E on Cyclophosphamide-Induced Cardiotoxicity in Male Rats. Biomedicines, 11(2), 390, 1-14. https://doi.org/10.3390/biomedicines11020390.
  32. Iqubal, A., Iqubal, M. K., Sharma, S., Ansari, M. A., Najmi, A. K., Ali, S. M., Ali, J., & Haque, S. E. (2019). Molecular mechanism involved in cyclophosphamide-induced cardiotoxicity: Old drug with a new vision. Life Sciences, 218, 112–131.
  33. Meng, E., Hsu, Y., & Chuang, Y. (2018). Advances in intravesical therapy for bladder pain syndrome (BPS)/interstitial cystitis (IC). LUTS: Lower Urinary Tract Symptoms, 10(1), 3–11.
  34. Brossard, C., Lefranc, A.-C., Pouliet, A.-L., Simon, J.-M., Benderitter, M., Milliat, F., & Chapel, A. (2022). Molecular Mechanisms and Key Processes in Interstitial, Hemorrhagic and Radiation Cystitis. Biology, 11(7), 972, 1-18. https://doi.org/10.3390/biology11070972.
  35. Sherif, I. (2020). Uroprotective mechanisms of natural products against cyclophosphamide-induced urinary bladder toxicity: A comprehensive review. Acta Scientiarum Polonorum Technologia Alimentaria, 19(3), 333–346.
  36. Jaber, F. A., & Tawfeek, S. El. (2023). The Potential Ameliorative Effect of Bone Marrow Derived Mesenchymal Stem Cells on Cyclophosphamide Injured Lung in Adult Female Albino Rats. Egyptian Journal of Histology, 46(1), 448–459.
  37. Almeida de Oliveira, L. S., de Moura Bandeira, S. R., Gomes Gonçalves, R. L., Pereira de Sousa Neto, B., Carvalho de Rezende, D., dos Reis-Filho, A. C., Sousa, I. J. O., PinheiroNeto, F. R., Timah Acha, B., & do Nascimento Caldas Trindade, G. (2022). The isopropyl gallate counteracts cyclophosphamide-induced hemorrhagic cystitis in mice. Biology, 11(5), 728, 1-17. https://doi.org/10.3390/biology11050728.
  38. Kassab, A. A., & Tawfik, S. M. (2018). Effect of a caffeinated energy drink and its withdrawal on the submandibular salivary gland of adult male albino rats: A histological and immunohistochemical study. Egyptian Journal of Histology, 41(1), 11–26.
  39. Abonar, M., Aboraya, A., Elbakary, N., & Elwan, W. (2022). Effect of Energy Drink on the Pancreas of Adult Male Albino Rat and the Possible Protective Role of Avocado Oil. Histological and Immunohistochemical Study. Egyptian Journal of Histology, 45(2), 386–403.
  40. Omar, N. M., & Sarhan, N. R. (2017). The possible protective role of pumpkin seed oil in an animal model of acid aspiration pneumonia: Light and electron microscopic study. Acta Histochemica, 119(2), 161–171.
  41. Hamdi, H. (2020). Testicular dysfunction induced by aluminum oxide nanoparticle administration in albino rats and the possible protective role of the pumpkin seed oil. The Journal of Basic and Applied Zoology, 81, 1–12.
  42. Kassab, A. A., Moustafa, K. A. A., & Abd-El-Hafez, A. A. (2020). The possible protective role of pumpkin seed oil in ameliorating tongue mucosal damage induced by orlistat in adult male albino rats: A light and scanning electron microscopic study. Egyptian Journal of Histology, 43(4), 975–987.
  43. Ofoego, U. C., Nweke, E. O., & Orji, C. (2019). Co-Administration of Cyclophosphamide and Ethanolic Seed Extract Of Telfairia Occidentalis (Pumpkin) Protects Testicular Functions In Adult Male Albino Wistar Rats. World Journal of Pharmaceutical Research, 8(3), 325–342.
  44. Andrade, I. G. A., Suano-Souza, F. I., Fonseca, F. L. A., Lago, C. S. A., & Sarni, R. O. S. (2021). Selenium levels and glutathione peroxidase activity in patients with ataxia-telangiectasia: association with oxidative stress and lipid status biomarkers. Orphanet Journal of Rare Diseases, 16, 1–10.
  45. Bardaa, S., Ben Halima, N., Aloui, F., Ben Mansour, R., Jabeur, H., Bouaziz, M., & Sahnoun, Z. (2016). Oil from pumpkin (Cucurbita pepo L.) seeds: evaluation of its functional properties on wound healing in rats. Lipids in Health and Disease, 15, 1–12.
  46. Hussain, A., Kausar, T., Sehar, S., Sarwar, A., Quddoos, M. Y., Aslam, J., Liaqat, A., Siddique, T., An, Q. U., & Kauser, S. (2023). A review on biochemical constituents of pumpkin and their role as pharma foods; a key strategy to improve health in post COVID 19 period. Food Production, Processing and Nutrition, 5(1), 22, 1-14. https://doi.org/10.1186/s43014-023-00138-z.
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