EFFECT OF TOTAL AND PARTIAL SPLENECTOMY ON BACTERIAL CLEARANCE IN PRAZIQUANTEL TREATED AND UNTREATED SCHISTOSOMAL MICE
Mikilail, N., El Sherbini, E., El Mohammadi, H., Botros, S., El Attar, I. (2000). EFFECT OF TOTAL AND PARTIAL SPLENECTOMY ON BACTERIAL CLEARANCE IN PRAZIQUANTEL TREATED AND UNTREATED SCHISTOSOMAL MICE. EKB Journal Management System, 19(4), 361-366. doi: 10.21608/ejsur.2000.378225
N.E. Mikilail; E. El Sherbini; H. El Mohammadi; S. Botros; I. El Attar. "EFFECT OF TOTAL AND PARTIAL SPLENECTOMY ON BACTERIAL CLEARANCE IN PRAZIQUANTEL TREATED AND UNTREATED SCHISTOSOMAL MICE". EKB Journal Management System, 19, 4, 2000, 361-366. doi: 10.21608/ejsur.2000.378225
Mikilail, N., El Sherbini, E., El Mohammadi, H., Botros, S., El Attar, I. (2000). 'EFFECT OF TOTAL AND PARTIAL SPLENECTOMY ON BACTERIAL CLEARANCE IN PRAZIQUANTEL TREATED AND UNTREATED SCHISTOSOMAL MICE', EKB Journal Management System, 19(4), pp. 361-366. doi: 10.21608/ejsur.2000.378225
Mikilail, N., El Sherbini, E., El Mohammadi, H., Botros, S., El Attar, I. EFFECT OF TOTAL AND PARTIAL SPLENECTOMY ON BACTERIAL CLEARANCE IN PRAZIQUANTEL TREATED AND UNTREATED SCHISTOSOMAL MICE. EKB Journal Management System, 2000; 19(4): 361-366. doi: 10.21608/ejsur.2000.378225

EFFECT OF TOTAL AND PARTIAL SPLENECTOMY ON BACTERIAL CLEARANCE IN PRAZIQUANTEL TREATED AND UNTREATED SCHISTOSOMAL MICE

The Egyptian Journal of Surgery
Volume 19, Issue 4, October 2000, Page 361-366  XML PDF (183.95 K)
Document Type: Original Article
DOI: 10.21608/ejsur.2000.378225
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Authors
N.E. Mikilail* 1; E. El Sherbini2; H. El Mohammadi3; S. Botros4; I. El Attar5
1Departments of Surgery, Theodor Bilharz Research Institute. Division of Statistics and Epidemiology National Cancer Institute
2Departments of Bacteriology, Theodor Bilharz Research Institute. Division of Statistics and Epidemiology National Cancer Institute
3Departments of Biochemistry, Theodor Bilharz Research Institute. Division of Statistics and Epidemiology National Cancer Institute
4Departments of Pharmacology, Theodor Bilharz Research Institute. Division of Statistics and Epidemiology National Cancer Institute
5Division of Statistics and Epidemiology National Cancer Institute
Abstract
Splenectomy for trauma or haematological diseases, affects the bacterial clearance as well as the immunological status 
of the patient. Over-whelming postsplenectomy sepsis syndrome carries 50-80% mortality. The ability of the spleen, liver and kidney to filter inoculated Staphylococcs aureus and the serum staphylococcal anti-DNase, was investigated in totally and partially splenectomized Schistosoma mansoni infected mices (n=120). Group Gl (n=60) was treated with the 
antischistosomal praziquantel six weeks post infection, while G2 (n=60) was left untreated control. Two weeks later, total 
(G1T and G2T), 75% partial (G1P and G2P) or sham splenectomy (G1C and G2C) were respectively performed. After four 
weeks, all animals were inoculated with ATCC 25923 Staphylococc aureus (3 x 107 C.F.U/ml saline/mouse). Twenty-four 
hours later, the liver, spleen, kidney, and serum were collected for bacterial counting and serum staphylococcal anti-Dnase testing. The Student's t, Wilcoxon two-sample and ANOVA tests were used for the statistical analysis. A significantly higher bacterial counts in the splenic remnant of G1P vs. G1C, and in the liver and kidney of G1T vs. G1C was found. Serum Staphylococcal anti-DNase level was more evident in G1 at almost the same mean log value of 0.6 and 0.5 but at different percentages of positivety (G1T=63 .6%, GlP=42.8%, G1C=29.4%). In conclusion, in the treated Schistosoma infected mice, the spleen and even a part of it, is an efficient bacterial filter and partial splenectomy may be preferred than total splenectomy.
Praziquantel treatment favored the host's power to filter bacteria and to produce antibody.
Keywords
splenectomy; post splenectomy syndrome; bacterial inoculation; schistosomiasis
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