Comparison between miR-199b-5p and AFP in the diagnosis of Hepatitis C-associated hepatocellular carcinoma | ||
| Azhar International Journal of Pharmaceutical and Medical Sciences | ||
| Article 8, Volume 5, Issue 1, January 2025, Pages 99-108 PDF (464.77 K) | ||
| Document Type: Original research articles | ||
| DOI: 10.21608/aijpms.2024.214335.1219 | ||
| Authors | ||
| Noha Osama* 1; Noha N Amer2; Amal A Mohamed3; Mohamed Salah4; Mohamed R Ezz Al Arab5; Rehab R El-Awady2 | ||
| 1Pediatritic Nutrition, Fitoverfat Nutrition Clinic | ||
| 2Department of Biochemistry and Molecular Biology, Faculty of pharmacy (Girls) Al-Azhar University Cairo, Egypt | ||
| 3Department of Biochemistry and Molecular Biology, National Hepatology, and Tropical Medicine Research Institute | ||
| 4Department of Internal Medicine, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt | ||
| 5Department of Hepatology, Ahmed Maher Teaching Hospital, Egypt | ||
| Abstract | ||
| Background: Hepatocellular carcinoma (HCC) is a global burden. Early detection through surveillance methods has increased patient survival. The miR-199b-5p could be a powerful biomarker for HCC detection. Therefore, this study aimed to estimate the diagnostic value of miR-199b-5p as an accurate non-invasive diagnostic marker for early diagnosis of hepatitis C virus (HCV)-induced HCC compared to Alpha-fetoprotein (AFP). Patients and Methods: 300 participants were enrolled in this study and categorized into three main groups:100 (HCV), 100 (HCC), and 100 healthy controls. The expression profile of miRNA-199b-5p was analyzed using Real-time polymerase chain reaction (real-time PCR) technology for all participants enrolled in this study. Results: miRNA-199b-5p had more diagnostic performance in differentiating the HCC group from the control group, showing 72% sensitivity and 62% specificity (AUC:0.770) in comparison to AFP, which had less significant diagnostic performance in differentiating the HCC group from the control group. Showing 67% sensitivity and 51% specificity (AUC:0.675). Conclusion: The results indicated a higher diagnostic value of miRNA-199b-5p than AFP in detecting HCC patients. Thus, miRNA-199b-5p could be a diagnostic biomarker for HCC with superiority over AFP. | ||
| Keywords | ||
| HCC; HCV; miRna-199b-5p; AFP | ||
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