PRODUCTION OF MONOCLONAL ANTIBODIES AGAINST BOVINE PARVOVIRUS | ||
Veterinary Medical Journal (Giza) | ||
Volume 45, Issue 4, October 1997, Pages 449-455 PDF (3.19 M) | ||
Document Type: Original Article | ||
DOI: 10.21608/vmjg.1997.378369 | ||
Authors | ||
MERVAT MAHMOUD1; IKRAM KARIM1; M SHALABY2 | ||
1Animal Health Research Institute, Dokki, Giza, Egypt. | ||
2Dept. of Virology, Faculty of Vet. Med., Cairo University, 12211 Giza, Egypt. | ||
Abstract | ||
Monoclonal antibodies (MAbs) against bovine Parvovirus (BPV) were successfully produced after fusing the spleen cells of an immunized mouse with myeloma cells. A total of six hybridomas were found to produce MAbs against BPV with different” reactivilles Using heamagglutination test. these clones are: 3B10, 3c5,3D6, 2H6 and 1E6. the first three clones produced MAbs of IgG class, but the last three clones produced those of the IgM class and all of them contained K light chain. | ||
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