Insight on Circulating miRNA-106b and miRNA-198 Biomarkers for Prediction of HCC in Patients with HCV-Induced Liver Diseases | ||||
| Biochemistry Letters | ||||
| Volume 20, Issue 1, 2024, Page 106-122 PDF (844.01 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/blj.2024.297500.1058 | ||||
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| Authors | ||||
Othman Ali   1; Hisham Ismail 2; Amal A. Mohamed3; Alshaimaa F Hegazy4
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| 1Chemistry department (Biochemistry Division)-Faculty of Science-Minia University-ElMinia-Egypt | ||||
| 2Biochemistry Division, Chemistry Dept., Faculty of Science, Minia University, Minia, Egypt | ||||
| 3National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt | ||||
| 4Chemistry department- Faculty Of Science – Minia University | ||||
| Abstract | ||||
| Background: Many circulating microRNAs have been studied and analyzed for non-invasive prediction of hepatocellular carcinoma in HCV-infected Egyptians. However, both miR-106b and miR-198 have not received the adequate concern for research. Aim of study: The present study sought to create a novel model for diagnosis and predication of HCC using miRNA-106b and miR-198. Material & Methods: 100 patients with HCV-induced liver diseases and 32 normal volunteers as controls were included. The liver diseases include chronic hepatitis C (n = 32), liver cirrhosis (n=36), and HCC (n = 32). Real-time-PCR (qRT-PCR) used to asses serum microRNA-106b and microRNA-198 expression levels. Level of several traditional markers of liver function and ∝-fetoprotein were determined. Analysis data was determined using SPSS 20 and area under receiver operating characteristic curve utilized to assess variables diagnostic power. Results: The fold change of miR-106b expression (Median; IQR) was significantly (P < 0.0001) upregulated in HCC patients in comparison with LC patients and CHC patients. However, miR-198 expression was decreasing gradually with the progression of liver disorder from CHC to LC to HCC. The levels of AFP and other traditional HCC-associated markers showed significant differences (p < 0.05) between the investigated groups. The AUROC analysis showed that both miR-106b and miR198 had higher diagnostic values (AUROC = 0.920 and 0.888; respectively) than AFP (AUROC = 0.847) to differentiate between HCC and non-HCC subjects. Conclusion: miR-106b and miR-198 expression levels of can be utilized as non-invasive sensitive and specific biomarkers for early prediction of HCV-induced HCC in Egyptian patients. | ||||
| Keywords | ||||
| miR-106b; miR-198; Hsp27; P53; HCC | ||||
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