POLYMERASE CHAIN REACTION AND ADAPTED ENZYME LINKED IMMUNOSORBENT ASSAY FOR DIAGNOSIS OF CAMEL BRUCELLOSIS | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 55, Issue 4, October 2007, Page 1067-1075 PDF (3.68 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2007.378787 | ||||
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| Author | ||||
| M AL-DUBAIB* | ||||
| Dept. of Veterinary Medicine, College of Agriculture and Veterinary Medicine Qassim University, P. O. Box 6622, Buraydah 51452, Qassim, KSA | ||||
| Abstract | ||||
| In the present study, polymerase chain reaction was carried out on 10 brucella isolates recovered from camels affected with brucellosis central region of Saudi Arabia. Brucella abortus as well as Brucella melitensis specific primers were employed for the assay. All isolates were identified as B. melitensis. This was in agreement with the results of the traditional bacteriological identification. Moreover, antibodies against camel IgG was raised in rabbits and purified with polystyrene affinity chromatography. The purified anti-camel IgG was conjugated with horseradish peroxidase (HRPO) using the sodium periodate method. The anticamel-HRPO conjugate prepared in this study was tested in an indirect ELISA adapted in the Same study on camel sera positive and negative for brucellosis as indicated by the Rose Bengal plate test. The conjugate was found efficient and was able to elucidate positive and negative samples at a dilution of 1/40. | ||||
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