Assessment of (EZH2) expression, digital image analysis of CD8+ lymphocytes, and Automated nuclear morphometry in renal cell carcinoma | ||||
| Benha Medical Journal | ||||
| Article 663, Volume 41, Issue 6, October 2024, Page 164-185 PDF (1.7 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bmfj.2024.298646.2107 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Eman M. Zedan1; ranih Z. Amer1; sara abd El Fattah madkour  2; Marwa S. Abd Allah3
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| 1Department of anatomic Pathology, Faculty of medicine, Banha University. | ||||
| 2department of pathology,faculty of medicine, banha univesity, banha, egypt | ||||
| 3Pathology Department, Faculty of Medicine, Benha University, Benha, Qalyubia, Egypt | ||||
| Abstract | ||||
| Background: The enhancer of zeste homolog-2 is overexpressed in many human cancers. High CD8+ T cell density was associated with favorable prognosis in various tumors, but this isn’t true for all cancers. Nuclear grading of RCC is an important prognostic factor but it’s subjective. Automated nuclear morphometry is more accurate. Aim: Evaluation of EZH2 expression, CD8+ density, and nuclear morphometry in RCC. Methods: In this retrospective study, IHC staining of EZH2 and CD8+ was done and assessed for each RCC case. CD8+ density was calculated. Quantitative nuclear morphometry was done by automated image analysis. Results: EZH2 expression showed a highly significant relation with histologic grade(P=0.002), tumor extent(P=0.012), and AJCC stage(P=0.004)& showed a significant relation with nodal metastasis (P=0.033), and distant metastasis(P=0.044). A highly significant relation between CD8+ density and histologic types(P=0.0001), histologic grade(P | ||||
| Keywords | ||||
| Renal cell carcinoma; EZH2; CD8+; Nuclear morphometry; image analysis | ||||
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