CHARACTERIZATION OF TRYPANOSOMA EVANSI ANTIGEN BY SDS-PAGE AND WESTERN BLOT | ||||
Veterinary Medical Journal (Giza) | ||||
Volume 56, Issue 2, April 2008, Page 63-69 PDF (2.96 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/vmjg.2008.378920 | ||||
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Authors | ||||
EL MAGHRBI, A A* 1; EL SAYED, I H1; HASSANEN, A S1; NISREEN EZELDIN MAHMOUD1; HOSNI, M M2 | ||||
1Dept. of Microbiology and Parasitology, Faculty of Vet. Medicine, Elfatah Uni. | ||||
2Dept. of Parasitology, Faculty of Vet. medicine, Cairo Uni. | ||||
Abstract | ||||
SDS-PAGE of T.evansi antigen after staining with commassie blue stain showed the presence of 26 bands ranging between 222.56 - 9.0 KDa. Western blot analysis revealed immunoprecipitation only with 2 proteins components with camels positive serum and had molecular weight of 98.976 and 16.678 KDa. While with mice positive sera revealed only one immuoprecipitation with protein component had molecular weight 16.678 KDa. | ||||
Keywords | ||||
Trypanosoma evansi; SDS-PAGE; Western blot; Camel | ||||
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