INVESTIGATION OF ANTICANCER MECHANISMS OF A CHALCONE/XANTHINE HYBRID IN NON-SMALL CELL LUNG CANCER

Elrehany, Omar Mahmoud; Ahmed, Al - Shaimaa Faissal; Abou-Zied, Hesham A.; Hayallah, Alaa M.; Abdel-Aziz, Mohamed; Nazmy, Maiiada Hassan

doi:10.21608/bfsa.2024.305647.2224

	INVESTIGATION OF ANTICANCER MECHANISMS OF A CHALCONE/XANTHINE HYBRID IN NON-SMALL CELL LUNG CANCER
Bulletin of Pharmaceutical Sciences Assiut University
Volume 47, Issue 2, December 2024, Page 1353-1366 PDF (887.13 K)
Document Type: Original Article
DOI: 10.21608/bfsa.2024.305647.2224
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Authors
Omar Mahmoud Elrehany ¹; Al - Shaimaa Faissal Ahmed²; Hesham A. Abou-Zied ³; Alaa M. Hayallah ⁴^{, 5}; Mohamed Abdel-Aziz ⁶; Maiiada Hassan Nazmy¹
¹Department of Biochemistry, Faculty of pharmacy, Minia University, Minya, Egypt
²Department of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University
³Medicinal Chemistry Department, Faculty of Pharmacy, Deraya University, Minia, Egypt
⁴Pharmaceutical Organic Chemistry Department, Faculty of Pharmacy, Assiut University, Assiut 71526, Assiut, Egypt
⁵Pharmaceutical Chemistry Department, Faculty of Pharmacy, Sphinx University, New Assiut, Egypt
⁶Medicinal Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt
Abstract
Despite major advances in therapy, Lung cancer remains the major cause of death among cancer cases. Chalcone/xanthine Hybrid is a synthetic xanthine derivative that was found to exert an anticancer action in various cancer types. Thus, the current study sought to discover if the chalcone/xanthine hybrid influences the etiology of non-small cell lung cancer (NSCLC). We found that the chalcone/xanthine hybrid inhibited cell cycle progression while inducing apoptosis and causing arrest of the cell cycle in HOP-92 cells. Flow cytometry demonstrated that chalcone/xanthine hybrid strongly induced apoptosis in HOP-92 cells, as well as alterations in apoptosis-related protein expression, including an increase in Bax, caspase3, caspase8, caspase-9, P53 expression, as well as a decrease in Bcl-2 expression. Moreover, the chalcone/xanthine hybrid slowed cell cycle progression from the G1 phase to the S phase. Furthermore, the ratio of phosphorylated proteins (AKT, MEK, ERK½, and P38) to total protein quantity reduced, indicating regulation of downstream protein signaling pathways governing cell cycle growth. Overall, this chalcone/xanthine hybrid may be considered a therapeutic option for NSCLC via apoptosis induction.
Keywords
NSCLC (non-small cell lung cancer); HOP-92 (Hopkins-92); MAPK (mitogen activated protein kinase)


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