Biochemical and Functional Characterization of Kombucha tea or Probiotic bacteria and Their Preservative Action on Chicken Meat | ||||
| Egyptian Journal of Nutrition | ||||
| Article 4, Volume 39, Issue 3 - Serial Number 1, September 2024, Page 43-59 PDF (1.21 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/enj.2024.379587 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Rehab Ahmed11; Eweda Wedad; Amin1 Shimaa; Fahmy2 Gehan2 | ||||
| 11- Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams University, P.O. Box 68,adayek Shubra, 11241 Cairo, Egypt | ||||
| 22- Department of Infection Prevention and Control, Ain Shams University Specialized Hospital, El-Khalifa El-Maamoun, Qobba Bridge, Heliopolis, P.O. Box 4393002, Cairo, Egypt. | ||||
| Abstract | ||||
| ABSTRACT Antibiotic residues in chicken meat pose significant health risks, including direct toxicity, antibiotic resistance, gut microbiota disruption and mutagenesis. The aim of this study is to detect the antibiotic residues in meat samples of chicken, evaluate the thermal stability of these residues, and investigate if the kombucha can be used as a growth promoter instead of veterinary antibiotics. Meat samples of total 41 chickens were collected randomly from various slaughterhouses in Cairo. Qualitative analysis using the Agar diffusion method was performed and quantitative analysis for positive samples were estimated by LC–MS/MS. The thermal stability of antibiotics was tested by boiling at 100°C for 30 minutes and freezing for 4 weeks at -18°C. Antibiotic residues were detected in 16%, 5%, and 5% of total samples for chlortetracycline, ciprofloxacin, and sulphadiazine, respectively, and in 11% for other antibiotics. Out of the 41 samples, 19 (46%) were violative, exceeding the Maximum Residue Limits set by the European Union. Boiling for 30 minutes could effectively eliminate antibiotic residues but freezing for 4 weeks at -18°C did not. Kombucha demonstrated growth-promoting effects in chickens, with the best performance observed when adding 80 ml per 1000 ml of drinking water. | ||||
| Keywords | ||||
| Keywords: Antibiotic residues; Chicken meat; Detection LC-MS/MS; Growth-promoting antibiotics; kombucha | ||||
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