ASSESSMENT OF THE EFFICACY O SEPHADEX FILTRATION OF BUFFALO SPERMATOZOA FOR CRYOPRESERVATION | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 54, Issue 2, April 2006, Page 295-307 PDF (6.34 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2006.379755 | ||||
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| Authors | ||||
| MAHA ZIADA* 1; MONA ALAA EL-DEEN2; A ZAKY1; KARIMAN HASSANIN1 | ||||
| 1Artificial Insemination and Embryo Transfer Dept. | ||||
| 2Biology of Reproduction dept. Animal Reproduction Research Institute Al-haram, Giza, Egypt | ||||
| Abstract | ||||
| In the present study, two experiments were conducted. The first experiment aimed to study the influence of buffalo bull semen filtration throughout different grades of sephadex columns (G-25, G-50, G-75, G-100 and G-200) on semen quality. Semen samples were collected from four buffalo bulls, diluted 1:20 with Tris buffer, loaded in different grades of sephadex columns and kept for 45 minutes at 37eeC, Filtered semen samples showed significantly (p<0.0001) higher individual motility (68.75 - 84.38 %) in different grades of sephadex when compared to control samples (63.13%). Percentages of live spermatozoa were found to be higher for filtered samples (71.38 - 88.63%) than that of control samples (65.94%). Percentages of major and minor abnormal spermatozoa were found to be decreased (2.37 - 4.5% and 1.56 - 5.12%, respectively) significantly (p< 0.0001) than that of control samples (5.56 and 6.31%, respectively). Percentages of spermatozoa with acrosomal defects were found to be significantly (p<0.0001) in comparison w'th control samples (4.63%), The loss in sperm number (0.67 to 0.99 vs 1.25 X 109 /mi) could be adequately compensated by obtaining higher values of all other important semen quality traits. The use of G-75 sephadex grade is recommended for wider use because it gives a more balanced picture of semen quality compared with other grades studied, The second experiment aimed to study the efficacy of sephadex G-75 filtration of buffalo spermatozoa for cryopreservation. Semen samples were collected from the same bulls used in the first experiment. Split samples were diluted 1:20 with Tris buffer, filtered through sephadex G-75 columns and centrifuged at 1500 rpm for 5 minutes. The supernatant was discarded and the sediments were re-diluted and frozen in Obtidyl diluent (Tris based diluent). Filtration was found to improve semen quality after dilution, freezing and thawing. Post-dilution, filtrated sperm motility was significantly (p<0.0001) improved (86.25%) when compared with control samples (67.91%. Post-thaw sperm motility of filtered samples was significantly (p<0.001) higher (61.25%) than that of control samples (45.83%), Filtration was significantly enhanced sperm viability after thawing (p<0.0001), where viability Indices were averaged 1S1.71 vs 90.83 for control samples. Percentages of spermatozoa with acrosomal defects were significantly (p<0,0001) reduced for filtered samples after dilution (1.92 vs 5.83%) and after freezing and thawing (7.75 vs 14.66%). Bull individualities were found prominent for some parameters: post thaw sperm motility and viability indices (p<0.05). | ||||
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