ONE-STEP RT-PCR TRAGETING THE FUSION PROTEIN GENE FOR DETECTION OF BOVINE RESPIRATORY SYNCYTIAL VIRUS
ELERAKY, N., KANIA, S., POTGIETER, L. (2006). ONE-STEP RT-PCR TRAGETING THE FUSION PROTEIN GENE FOR DETECTION OF BOVINE RESPIRATORY SYNCYTIAL VIRUS. EKB Journal Management System, 54(3), 685-690. doi: 10.21608/vmjg.2006.379787
NASSER ELERAKY; STEPHEN KANIA; LEON POTGIETER. "ONE-STEP RT-PCR TRAGETING THE FUSION PROTEIN GENE FOR DETECTION OF BOVINE RESPIRATORY SYNCYTIAL VIRUS". EKB Journal Management System, 54, 3, 2006, 685-690. doi: 10.21608/vmjg.2006.379787
ELERAKY, N., KANIA, S., POTGIETER, L. (2006). 'ONE-STEP RT-PCR TRAGETING THE FUSION PROTEIN GENE FOR DETECTION OF BOVINE RESPIRATORY SYNCYTIAL VIRUS', EKB Journal Management System, 54(3), pp. 685-690. doi: 10.21608/vmjg.2006.379787
ELERAKY, N., KANIA, S., POTGIETER, L. ONE-STEP RT-PCR TRAGETING THE FUSION PROTEIN GENE FOR DETECTION OF BOVINE RESPIRATORY SYNCYTIAL VIRUS. EKB Journal Management System, 2006; 54(3): 685-690. doi: 10.21608/vmjg.2006.379787

ONE-STEP RT-PCR TRAGETING THE FUSION PROTEIN GENE FOR DETECTION OF BOVINE RESPIRATORY SYNCYTIAL VIRUS

Veterinary Medical Journal (Giza)
Volume 54, Issue 3, July 2006, Pages 685-690    PDF (1.38 M)
Document Type: Original Article
DOI: 10.21608/vmjg.2006.379787
Authors
NASSER ELERAKY* 1; STEPHEN KANIA2; LEON POTGIETER2
1Department of Virology, Faculty of Veterinary Medicine, Sadat City, Menoufia University
2Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, Konxville, Tennessee, USA.
Abstract
RT-PCR F targeting a fragment of the fusion protein (F) gene of bovine respiratory syncytial virus (BRSV) has been recommended to detect BRSV. This assay is used to be performed in two steps after RNA extraction: cDNA synthesis then PCR. In this study, cDNA synthesis and PCR have been performed in one step single tube. This modification may save time and effort with the same sensitivity and specificity.
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