ISOLATION AND IDENTIFICATION OF BOVINE EPHEMERAL FEVER VIRUS FROM CATTLE IN MIDDLE EGYPT
M, M., HEGAZI, A., EL-SHABRAWY, M., TAMAM, S., GAMIL, G. (2006). ISOLATION AND IDENTIFICATION OF BOVINE EPHEMERAL FEVER VIRUS FROM CATTLE IN MIDDLE EGYPT. EKB Journal Management System, 54(4), 877-886. doi: 10.21608/vmjg.2006.379911
MADBOULY M; A HEGAZI; MONA EL-SHABRAWY; S TAMAM; G GAMIL. "ISOLATION AND IDENTIFICATION OF BOVINE EPHEMERAL FEVER VIRUS FROM CATTLE IN MIDDLE EGYPT". EKB Journal Management System, 54, 4, 2006, 877-886. doi: 10.21608/vmjg.2006.379911
M, M., HEGAZI, A., EL-SHABRAWY, M., TAMAM, S., GAMIL, G. (2006). 'ISOLATION AND IDENTIFICATION OF BOVINE EPHEMERAL FEVER VIRUS FROM CATTLE IN MIDDLE EGYPT', EKB Journal Management System, 54(4), pp. 877-886. doi: 10.21608/vmjg.2006.379911
M, M., HEGAZI, A., EL-SHABRAWY, M., TAMAM, S., GAMIL, G. ISOLATION AND IDENTIFICATION OF BOVINE EPHEMERAL FEVER VIRUS FROM CATTLE IN MIDDLE EGYPT. EKB Journal Management System, 2006; 54(4): 877-886. doi: 10.21608/vmjg.2006.379911

ISOLATION AND IDENTIFICATION OF BOVINE EPHEMERAL FEVER VIRUS FROM CATTLE IN MIDDLE EGYPT

Veterinary Medical Journal (Giza)
Volume 54, Issue 4, October 2006, Page 877-886  XML PDF (2.55 MB)
Document Type: Original Article
DOI: 10.21608/vmjg.2006.379911
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Authors
MADBOULY M* 1; A HEGAZI2; MONA EL-SHABRAWY2; S TAMAM1; G GAMIL2
1Beni Suef Univeristy
2National Research Center
Abstract
During 2000-2001 outbreaks of bovine ephemeral fever (BEF) occurred at Middle Egypt, 251 heparinized blood samples from cattle and buffaloes with BEF symptoms were collected for virus isolation and identification and 549 serum samples were collected for serological examination. Suckling baby mice were intracerebrally (I/C) inoculation for virus isolation and identification, in the same time isolated virus was adapted to grow in both Verol21 and BKH21 cell line. Identification of isolated virus was achieved using both reference and prepared hyper immune serum against BEF virus in rabbit by using SPA agglutination test (SPA), Serum neutralization test (SNT), Mouse protection Test (MPT) and Agar gel precipitation test (AGPT).
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