PREPARATION OF INACTIVATED RABIES SUCKLING MOUSE BRAIN VACCINE ADJUVANTED WITH NATURAL IMMUNE STIMULANTS | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 54, Issue 4, October 2006, Pages 913-927 PDF (3.73 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2006.379914 | ||
| Authors | ||
| H MADBOULY* 1; A NOUR EL-DIEN2; DOSEILA ABEER2 | ||
| 1Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University | ||
| 2National Organization for Drug Control and Research, Cairo, Egypt | ||
| Abstract | ||
| The challenge virus standard (CVS-11) strain of fixed rabies virus was propagated on suckling mouse brains for several passages. These passages were titrated in 3-4 weeks old white Swiss mice using mouse inoculation test (MIT). The harvested infected brains of suckling mice were inactivated using 1/4000 beta-propiolactone (BPL) and adjuvanted using Nigella sativa oil and combination of vitamin E& Selenium. The prepared plain and adjuvanted rabies suckling mouse brain vaccines were subjected to quality control tests including its safety, sterility and potency using either National institute of Health (NIH) or immunogenicity test in mice. The obtained results were discussed and revealed that the adjuvanted mouse brain rabies vaccine with either nigella sativa or vitamin E and Selenium were highly immunogenic than the plain vaccine and can be used in human or animals safely. | ||
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