PREPARATION OF INACTIVATED RABIES SUCKLING MOUSE BRAIN VACCINE ADJUVANTED WITH NATURAL IMMUNE STIMULANTS
MADBOULY, H., NOUR EL-DIEN, A., ABEER, D. (2006). PREPARATION OF INACTIVATED RABIES SUCKLING MOUSE BRAIN VACCINE ADJUVANTED WITH NATURAL IMMUNE STIMULANTS. EKB Journal Management System, 54(4), 913-927. doi: 10.21608/vmjg.2006.379914
H MADBOULY; A NOUR EL-DIEN; DOSEILA ABEER. "PREPARATION OF INACTIVATED RABIES SUCKLING MOUSE BRAIN VACCINE ADJUVANTED WITH NATURAL IMMUNE STIMULANTS". EKB Journal Management System, 54, 4, 2006, 913-927. doi: 10.21608/vmjg.2006.379914
MADBOULY, H., NOUR EL-DIEN, A., ABEER, D. (2006). 'PREPARATION OF INACTIVATED RABIES SUCKLING MOUSE BRAIN VACCINE ADJUVANTED WITH NATURAL IMMUNE STIMULANTS', EKB Journal Management System, 54(4), pp. 913-927. doi: 10.21608/vmjg.2006.379914
MADBOULY, H., NOUR EL-DIEN, A., ABEER, D. PREPARATION OF INACTIVATED RABIES SUCKLING MOUSE BRAIN VACCINE ADJUVANTED WITH NATURAL IMMUNE STIMULANTS. EKB Journal Management System, 2006; 54(4): 913-927. doi: 10.21608/vmjg.2006.379914

PREPARATION OF INACTIVATED RABIES SUCKLING MOUSE BRAIN VACCINE ADJUVANTED WITH NATURAL IMMUNE STIMULANTS

Veterinary Medical Journal (Giza)
Volume 54, Issue 4, October 2006, Page 913-927  XML PDF (3.73 MB)
Document Type: Original Article
DOI: 10.21608/vmjg.2006.379914
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Authors
H MADBOULY* 1; A NOUR EL-DIEN2; DOSEILA ABEER2
1Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University
2National Organization for Drug Control and Research, Cairo, Egypt
Abstract
The challenge virus standard (CVS-11) strain of fixed rabies virus was propagated on suckling mouse brains for several passages. These passages were titrated in 3-4 weeks old white Swiss mice using mouse inoculation test (MIT). The harvested infected brains of suckling mice were inactivated using 1/4000 beta-propiolactone (BPL) and adjuvanted using Nigella sativa oil and combination of vitamin E& Selenium. The prepared plain and adjuvanted rabies suckling mouse brain vaccines were subjected to quality control tests including its safety, sterility and potency using either National institute of Health (NIH) or immunogenicity test in mice. The obtained results were discussed and revealed that the adjuvanted mouse brain rabies vaccine with either nigella sativa or vitamin E and Selenium were highly immunogenic than the plain vaccine and can be used in human or animals safely.
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