TYPING OF STREPTOCOCCI BY MEANS OF SPECIFIC ANTIBODY ADSORBED TO PROTEIN-A CONTAINING STAPHYLOCOCCI | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 38, Issue 1, January 1990, Pages 45-51 PDF (3.44 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.1990.380149 | ||
| Author | ||
| M. ISMAIL* | ||
| Department of microbiology, Faculty of Veterinary Medicine, Cairo University. | ||
| Abstract | ||
| Staphylococcus protein-A (SPA) is a cell wall consti- tuent of Staphylococcus aureus which has an extra - ordinary affinity for binding to the FC region of immunoglobulin G (IgG) from various mammalian species. Cell bound SPA is found to be useful as an adsorbent for detection of antigen-antibody complex (Goding, 1978, Kessler, 1981 and Lindmark et al., 1983). The precipitation test is widely used for typing of - streptococci by means of their carbohydrate substance or antigen "C" in their cell walls (Crusickshank et al., 1975). This test however, more laborious and time consuming as a routine test. A slide agglutina- tion method is here described, that renders strepto- coccal typing quick and easy to be performed. when specific anti-streptococcal antibodies, were added to a stabilized suspension of staphylococci , they became bound to the protein A on the cell wall via the FC structure of IgG (Kronvall and Williams ,1969 and Kronvall and Frommel, 1970); therefor ,orienta- ting the Fab-located antibody- combining sites outwards (Lind et al., 1972). When streptococci of the corresp onding group are added, coagglutinin occurs with the antibody coated staphylococci. Thus , this work was done to determine the more economic ,rapid and easy method for typing of the examined streptococci. | ||
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