DOT-BASED ENZYME IMMUNOASSAY FOR DETECTION OF CANINE PARVOVIRUS ANTIGEN (S) IN FECAL SAMPLES OF LIVING DOGS AND ORGANS OF DEAD AND STILLBIRTH PUPPIES | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 38, Issue 1, January 1990, Page 63-76 PDF (7.1 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.1990.380152 | ||||
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| Author | ||||
| AHMED EL-SANOUST* | ||||
| Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza-Egypt. | ||||
| Abstract | ||||
| Canine parvovirus is known as the etiological agent of haemorrhagic enteritis in dogs which sometimes | ends fataly in young puppies due to severe myocardi- tis (Appel and Carmichael, 1978; Peru et al., 1980; and Meyer, 1980). The infection of dogs with Canine Parvovirus (CPV) has been reported to be widely dist- ributed in many countries of the world like Holland (Osterhaus et al., 1980), Israel (Peru et al., 1980), West Germany (Klunker et al., 1983), USA (Kramer et al., 1980 and Carmichael et al., 1981), as well as Australia (Sabine et al., 1982), Costa Rica (Hernendez et al., 1984), India (Sherikar and Paranjape, 1985), and Nigeria (Kamalu, 1985). In Egypt CPV infection has been first reported to occur in police dogs only from clinical and histopathological findings (Bucci et al., 1982), In the last 2 decades, enzyme-linked immunosorbent assay has found its way in the field of rapid diagn- osis of a variety of animal diseases (Bommel,1983 and Charan and Guatam, 1984) bacause of its simpli- Cty, sensitivity and specificity over most other most other serological assays for antigen and antibody detection, The successful detection CPV anti- gen (s) in feces of infected dogs has also been | ||||
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