Determining the ability of mannoprotein extracted from Saccharomyces cerevisiae to cure some virulence factor genes of some species of pathogenic bacteria isolated from different sources | ||||
| Journal of Bioscience and Applied Research | ||||
| Volume 10, Issue 3, September 2024, Page 578-592 PDF (1.42 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jbaar.2024.381371 | ||||
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| Authors | ||||
| Ashwaq Muthanna Wahib; Mohammed Nadhir Maaroof | ||||
| College of Education for Pure Science, University of Tikrit, Iraq | ||||
| Abstract | ||||
| Background: Bacterial diseases play a major role in wound and burn infections and urinary tract infections, which lead to wound and burn contamination due to the change in the host defense mechanism. Objectives: Discovery of the ability of mannoprotein to cure the virulence factors genes of the causative agent of wound infections, burns, and urinary tract infections at the molecular level. Materials and methods: One hundred samples were collected from different sources of infections, and then cultured on blood agar, MacConkey agar, and mannitol agar. Bacterial isolates were identified using biochemical tests and molecular detection of these factors using specific primers - ndvB, rsmA, fimH, ureR, ureD, and hlb. Results: The ureR, rsmA, fimH, ureD, ndvB, and hlb genes were found in all isolates (100%). After the samples were treated with the first and second mannoprotein extracted from Saccharomyces cerevisiae, the genes responsible for the production of the virulence factors biofilm, Hemolysin, and urease were curing from appearing and the production of virulence factors was inhibited because the DNA was destroyed which indicates that Saccharomyces cerevisiae is one of the beneficial substances for the body's immunity against bacterial diseases. Conclusion: A difference was observed in the gene expression of the genes responsible for the production of biofilm, Hemolysin, and urease virulence factors in the bacterial isolates before and after treatment with mannoprotein extraction. | ||||
| Keywords | ||||
| mannoprotein; Gene expression; virulence factors; PCR; Curing gene | ||||
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