Microbiological evaluation of biofilm formation on denture base materials made using computer aided design/computer aided manufacturing (CAD/ CAM) Technology. An in vitro comparative study.

El Morshedy, Nada Abd ElKader; Bahey, Marwa Gamal; El Hadad, Fatma Abd ElRazik

doi:10.21608/edj.2024.286927.3080

	Microbiological evaluation of biofilm formation on denture base materials made using computer aided design/computer aided manufacturing (CAD/ CAM) Technology. An in vitro comparative study.
Egyptian Dental Journal
Volume 70, Issue 4, October 2024, Page 3465-3476 PDF (903.35 K)
Document Type: Original Article
DOI: 10.21608/edj.2024.286927.3080
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Authors
Nada Abd ElKader El Morshedy ¹; Marwa Gamal Bahey ²; Fatma Abd ElRazik El Hadad ³
¹Lecturer, Department of prosthetic dentistry. Faculty of Dentistry. Tanta University. Egypt
²Lecturer, Department of Microbiology & Immunology. Faculty of Medicine. Tanta University Egypt.
³Lecturer, Department of prosthetic dentistry. Faculty of Dentistry. Tanta university Egypt.
Abstract
Aim of the work: The purpose of this study was to compare the amount of adherent Candida albicans and Staphylococcus aureus on different denture base materials formed by CAD/CAM technology. Materials and Methods: The study was performed on 90 controlled type II diabetic patients from Prosthodontics Department for isolation of Staphylococcus aureus and Candida albicans, then detection of biofilm producing isolates and assess biofilm formation on different denture base materials formed by CAD-CAM technology. Forty disc-shaped (PMMA) samples were divided into two groups as follow: • Twenty 3D printed (PMMA) disc-shaped samples constructed by CAD/CAM technology. • Twenty milled (PMMA) disc-shaped samples constructed by CAD/CAM technology. The adherent cells and formation of Candida albicans and Staphylococcus biofilm were measured by using a microplate reader. At the end of the study all data were collected, tabulated, and statistically analyzed by IBM-SPSS statistics software. Results: In the current study biofilm formation on 3D printed specimens was more than milled specimens regarding Candida albicans, but there was no significant difference in both groups regarding Staphylococcus aureus. Conclusions: Additive 3D-printing technology resulted in increased microbial biofilm formation compared to CAD/CAM milling techniques on acrylic denture base resin.
Keywords
milling techniques; additive 3D-printing; biofilm formation


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