Screening and production of microbial L-arginase enzyme as anticancer agent from different soil environments in Egypt | ||||
| Ain Shams Medical Journal | ||||
| Articles in Press, Accepted Manuscript, Available Online from 01 October 2024 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/asmj.2022.146268.1029 | ||||
 View on SCiNiTO | ||||
| Author | ||||
Mohammed M Kassab  
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| Instructor of microbiology,immunology,faculty of pharmacy,Cairo university,Egypt Specialist of pharmacology, toxicology and clinical pharmacy. | ||||
| Abstract | ||||
| Background Arginine degrading enzymes are necessary for the treatment of auxotrophic cancers such as hepatic carcinoma,melanoma and colorectal cancers.Auxotrophic cancers have not the ability to synthesize argenine but normal cells can synthesize it.Argenine degrading enzymes include arginine deiminase, L-arginase enzyme and arginine decarboxylase enzyme. The aim of the study Production of microbial L-arginase enzyme as anticancer agent from different soil environments in Egypt. Type of study Screening experimental study Methodology our study were concerned with isolation and screening of bacterial producing L-arginase enzyme as anticancer agent for treatment of auxotrophic cancers from different soil environments.Also, concerned with determination of environmental and physiological factors affecting the growth of some L-arginase producing bacterial isolates.Characterization of L-arginase enzyme was also included in our study. In our study some bacterial isolates were analyzed for production of L-arginase on mineral arginine agar selective media(MAA). Results and discussion These bacterial isolates which showed positive growth on MAA utilized the arginine as the sole metabolic source of carbon and nitrogen for their growth.The optimal environmental and physiological factors affecting growth of these positive isolates were PH 7.3 at temperature 37c in aerobic conditions. The morphological and the biochemical tests revealed that Bacillus subtilis and Bacillus anthracis were the major positive bacterial producing L-arginase isolates of soil samples collected from different soil environments. Direct Nesslerization test was used for detection and screening of the presence of bacterial argenine degrading enzymes via their ability to produce ammonia from degrading the argenine in different dilutions of soil samples. | ||||
| Keywords | ||||
| Microbial L-arginase; production; anticancer agent | ||||
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