Effect of Chitosan Nanoparticles on Ram Sperm Characterisation after Semen Cryopreservation | ||
| Egyptian Journal of Veterinary Sciences | ||
| Article 8, Volume 56, Issue 12, December 2025, Pages 3121-3131 PDF (1.28 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2024.303143.2249 | ||
| Authors | ||
| Ayat abd Elmaqsoud Elshamy* 1; Ahmed M. Elroby1; Magdy R. Badr1; Mohamed M. Assi2; Heba F. Hozyen3; Eman M. Abd El Fattah4 | ||
| 1Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute (ARRI), Agricultural Research Center (ARC), AL haram, Giza, Egypt. | ||
| 2Reproductive Pathology Department, Animal Reproduction Research Institute (ARRI), Agricultural Research Center (ARC), AL haram, Giza, Egypt. | ||
| 3Animal Reproduction and A.I. Department, Veterinary Research Institute, National Research Centre (NRC), 33 El-Buhouth St., Dokki, Giza, Egypt. & Veterinary Physiology and Biochemistry Department, Faculty of Veterinary Medicine, Ain-Shams University, Egypt. | ||
| 4Biotechnology Research Unit, Animal Reproduction Research Institute (ARRI), Agricultural Research Center (ARC), AL haram, Giza, Egypt. | ||
| Abstract | ||
| This study evaluated the possible protective role of chitosan nanoparticles (CNPs) on ram semen after cryopreservation using a Tris-egg yolk-based extender. Chitosan synthesis and characterization were done by transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive analysis of X-ray spectroscopy (EDAX). Semen ejaculates were collected by artificial vagina from five rams with proven fertility (two ejaculates / ram / week) for five weeks. Only good quality ejaculates were pooled and then diluted with tris extender supplemented with different concentrations of CNPs (0, 5, 15, 25, 50, and 75 μg/ml). The diluted Samples gradually cooled to 4°C over 90 min, then equilibrated for farther 2hrs in a cold Cabinet (4°C) after that packed in 0.5ml French straws, frozen on liquid nitrogen (LN) vapor for 15 minutes then stored in LN until evaluation. Post-thawed samples were examined by computer-assisted sperm analysis (CASA) recording sperm motion parameters. Additionally, the acrosome defects, membrane integrity, antioxidant activity as well as sperm ultrastructure, and DNA integrity were recorded. The results showed a significant improvement in total, rapid, and progressive motility, in addition to preserving the integrity of the acrosome, the plasma membrane, and the ultrastructure of sperm after adding (15 µg/ml) of chitosan nanoparticles. There was a non-significant increase in antioxidant activity with a decrease in the levels of lipid peroxides and no adverse effect on the DNA. It could be concluded that CNPs cross-linked with dextran sulfate were able to successfully protect the viability of frozen-thawed ram sperm by decreasing cryo-injury. | ||
| Keywords | ||
| Chitosan Nanoparticles; Cryopreservation; Oxidative Stress; Sperm Ultrastructure; DNA; Ram | ||
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