Steroid Hormones and Inflammatory Markers in the Uterine Tissue and Follicular Fluid of Cattle and Buffalo with Uterine Bacterial Infection

Soror, Ashraf Hasan; S. Kotp, Mohamed; Sayed, Mohamed Aly; el-gabry, mahmoud abbas; Fouad, Ehab; El-Debaky, Hazem Ahmed; Abo El-Maaty, amal Mahmou; Abd El-Razik, Khaled A.; Desouky, Hassan M. Mohamed

doi:10.21608/ejvs.2024.311068.2304

	Steroid Hormones and Inflammatory Markers in the Uterine Tissue and Follicular Fluid of Cattle and Buffalo with Uterine Bacterial Infection
Egyptian Journal of Veterinary Sciences
Articles in Press, Corrected Proof, Available Online from 02 October 2024 PDF (2.36 MB)
Document Type: Original Article
DOI: 10.21608/ejvs.2024.311068.2304
View on SCiNiTO
Authors
Ashraf Hasan Soror ¹; Mohamed S. Kotp²; Mohamed Aly Sayed ¹; mahmoud abbas el-gabry ³; Ehab Fouad ⁴; Hazem Ahmed El-Debaky ¹; amal Mahmou Abo El-Maaty ²; Khaled A. Abd El-Razik⁵; Hassan M. Mohamed Desouky ¹
¹National Research Centre
²Animal Reproduction and Artificial Insemination department, Veterinary Research Institute, National Research Centre, Cairo, Egypt
³animal reproduction and artificial insemination, veterinary research institute, national research Centre, Giza, Egypt
⁴Dep of Zoonosis
⁵Animal Reproduction and Artificial Insemination department, Veterinary Research Institute, National Research Centre, Cairo, Egypt.
Abstract
Endometritis is the most common reproductive infertility problem in dairy animals. This study aimed to investigate the relation between the histopathological characterization of endometritis and ovarian steroid hormones in both uterine tissue and follicular fluids (FF) in addition to the proinflammatory cytokines and myeloperoxidase (MPO). Uteri collected from slaughter houses were subjected to hematoxylin and eosin (H&E) routine staining and Antisyndecan-1 immunohistochemistry aids to classify the type of endometritis. Bacteria in the apex of the examined uterine horns were isolated and identified. FF and uterine tissues homogenate were used to assay E2, progesterone (P4), tumor necrosis factor α (TNFα) and MPO. Results showed that sample-species type (cow uteri, buffalo uteri, cow FF, and Buffalo FF fluids) influenced (P<0.0001) MPO, E2, and P4. Uterine (Ut) pathology (acute suppurative, acute lymphocytic, chronic suppurative, chronic metritis, and normal); the isolated bacteria; Ut Pathology × Sample-Species; Ut Pathology × bacteria; and Bacteria ×Sample-Species influenced (P<0.0001) TNFα, MPO, E2, and P4. Ut Pathology × bacteria × Sample-Species affected TNFα, MPO, and P4. In conclusions, Immunohistochemistry is a reliable method to confirm the chronicity of endometritis. Both TNFα and MPO would be reliable markers for determining endometritis. Endometritis likely altered the FF proinflammatory markers and the steroid hormones and sequentially the future fertility. Buffaloes seem sensitive to uterine pathology caused by bacteria.
Keywords
Endometritis; Immunohistochemistry; MPO; Ovarian hormones; tumor necrosis factor α


Statistics Article View: 183 PDF Download: 252