Investigation of anti-oxidant activities of pyrazole based-compounds in a mice model of rheumatoid arthritis | ||
| Minia Journal of Medical Research | ||
| Articles in Press, Accepted Manuscript, Available Online from 07 October 2024 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjmr.2024.312272.1786 | ||
| Authors | ||
| Amany Haroun Abdel Naiem* 1; Ahlam M. Abdallah1; Salama R Abdelraheim1; Hend Mohammed Abdelghany1; Omar M. Mohafez2 | ||
| 1Department of Medical Biochemistry, Faculty of Medicine, Minia University, Minia 61511, Egypt | ||
| 2Department of Biochemistry, faculty of pharmacy, Al-Azhar University, Assiut-branch 71524, Egypt | ||
| Abstract | ||
| Rheumatoid arthritis (RA) is an autoimmune disease that causes joint inflammation and cartilage damage. Free radicals can harm the joint cartilage. Pyrazole compounds have variety of medical applications, including anti-inflammatory properties. Purpose of the study: The purpose of the study was to compare the effect of pyrazole derivatives on catalase, GSH, and NO levels in a mouse model of RA to indomethacin as a reference medicine in terms of oxidative stress. Basic procedures: Rheumatoid arthritis mouse model was created by immunization of DBA/1J mice with chicken type-II collagen and complete and incomplete Freund's adjuvants. Mice were placed into two groups, group I: control and group II: Rheumatoid arthritis mice: Mice were injected intradermal at the base of their tails with 100 μl chicken type II collagen emulsified in CFA and received a booster intradermal shot after three weeks of 100 μl l of chicken type II collagen emulsified in IFA. Mice with arthritis were categorised and treated as follows: Indomethacin group; treated with 1 mg/kg SC of indomethacin daily for five days. The M1E group; received M1E 5mg/kg SC daily for five days and fifteen days respectively. The M1G group received M1G 5mg/kg SC daily for five days and fifteen days respectively. Main findings: Pyrazole derivatives M1E and M1G dramatically improved catalase and GSH levels and decreased NO levels in arthritic mice. Principle conclusion: Two pyrazole derivatives, M1E and M1G, were shown to alleviate oxidative stress by increasing catalase and GSH activities and suppressing NO levels in RA mice. | ||
| Keywords | ||
| Rheumatoid arthritis (RA); Catalase (CAT); pyrazole derivatives; Glutathione (GSH); nitric oxide (NO) | ||
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