IMMUNOLOGICAL STUDIES ON PORIN PROTEINS OF PASTEURELLA HEAMOLYTICA IN GOAT | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 53, Issue 2, April 2005, Page 209-219 PDF (3.67 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2005.384673 | ||||
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| Authors | ||||
| SAHAR R MOHAMED* 1; HODA ABD EL-MONEM2 | ||||
| 1Bacteriology Department. Biotechnology Department | ||||
| 2Animal Health Research Institute, Doki, Giza, Egypt | ||||
| Abstract | ||||
| This study was carried out on 120 goats, 45 of them suffering from pneumonia with its clinical symptoms, 55 of them were died and 20 were apparently healthy. The morbidity and mortality rates reached 22.5% and 27.5%, respectively. A total of 25 nasal swabs were collected from pneumonic goat for isolation and identification of the causative agent. P. haemolytica was isolated pure in 44%. Other bacteria isolated coupled with Staphylococcus aureus in 24% and 32% Esherichia coil. The protein profiles of the purified bacteria was carried out using SDS poly acrylamide gel electrophoresis by comparing with molecular weight marker changed from 10 - 250 KDa. This study was conducted to characterize and identify the purity of major porin proteins in outer membrane with molecular weight of 38 - 42 kDa . Of pasteurella heamolytica usinb SDS-PAGE. In the active immunization assay, porin proteins 501.1g conferred 100% protection to challenged mice. In addition crud extract give 70% protection when challenged mice. Immunization with porin proteins of P.heaniolytica developed a significant increase in scrum antibody titre, as shown by enzyme linked immunasorbent assay., Stability assay , and opsonophagocytic - killing assay. | ||||
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