INCIDENCE AND SDS -PAGE PROTEIN PROFILE ANALYSIS FOR MYCOPLASMA SPP, AND ACHOLEPLASMA SPP. ISOLATED FROM CAMELS IN EGYPT | ||||
Veterinary Medical Journal (Giza) | ||||
Volume 53, Issue 2, April 2005, Page 231-239 PDF (3.24 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/vmjg.2005.384675 | ||||
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Authors | ||||
KAMELIA M OSMAN* 1; MONA I EL-ENBAAWY1; H Amer2 | ||||
1Department of Microbiology, Faculty of Veterinary Medicine, Cairo University. | ||||
2Animal Health Research Institute, Dokki ,Cairo | ||||
Abstract | ||||
Thirty-nine strains of Mycoplasma spp. (33) and Acholeplasma .spp. (6) were isolated from 118 pneumonic lungs and 110 tracheal samples obtained from different abattoirs in Egypt. The percentages of their isolaion were 8.3 % from pneumonic lungs and 8.7 % from tracheal samples. Based on the biochemical profile and growth inhibition results, the camel isolates were identified as, M. hovis, glucose positive mycoplasma and arginini. The identity of these isolates was further confirmed by growth inhibition test using a panel of specific antisera against selected reference Mycoplasma spp. Whole-cell protein patterns generated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) were used to identify and classify field isolates of a chosen Mycoplasma spp (19) and Acholeplasma spp (6). A high degree of similarity between most of the strains was established with strain-to-strain differences. | ||||
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