IMPORTANCE OF BACTERIOCIN OF LACTOBACILLUS ACIDOPHILUS IN PREVENTION , COLONIZATION AND PROLIFERATION OF ENTERIC BACTERIA IN CATTLE | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 53, Issue 2, April 2005, Page 241-249 PDF (2.99 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2005.384676 | ||||
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| Authors | ||||
| SAHAR MOHAMED* ; AZZA FARAG | ||||
| Bacteriology Department Animal Health Research Institute Dokki, Cairo, Egypt | ||||
| Abstract | ||||
| Bacteriocin produced by Lactobacillus acidophilus was tested for it's potential as aprobiotic cullure, it's exhibited good sensitivity to heat, pro- tease enzyme, acid tolerance, as well as bile resistance in media containing 0.3% bile acids. Bacteriocin was active over wide pH range and inhibited a number of pathogenic bacteria. The SDS - PAGE of the active fraction resulted in a single band with molecular mass of 5.4 KDa. In challenge test. Mice that were fed on milk based diets contain bacteriocin (108 C.F.U).showed 100%. protection level . Meanwhile the antibody titers in the serum of immunized mice with bacteriocin were significantly higher than the titers in the control of unprotected. mice . These results confirmed the probiotic effect of bacteriocin against colonization of most microorganism in the animal tissue as well as enhancing their immune response. | ||||
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