VIROLOGICAL AND PATHOLOGICAL STUDIES ON BUFFALO POX VIRUS ISOLATED FROM DAIRY BUFFALOES UDDER | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 53, Issue 2, April 2005, Page 267-278 PDF (5.34 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2005.384685 | ||||
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| Authors | ||||
| S SELIM* 1; MERVAT I ABD-EL-MONIEM2; NEBAL A HASSAN1 | ||||
| 1Pathology of Reproduction Department, Animal Reproduction Research Institute | ||||
| 2Virology Department, Animal Health Research Institute | ||||
| Abstract | ||||
| Pox like lesions were frequently recorded in 43.75% dairy buffaloes recently introduced into small and moderate dairy barns at Kafr EL-Gabal and Zawet Abo Mesalem districts at Giza Governorate. These affections led to reduction of milk yield. This work was done to isolate and identify the causative agent to overcome the problem and its economic losses. After inoculation of the prepared collected samples on the chorioallantoic membrane of embryonated chicken eggs, pock lesions were observed on the 4th or 5th day-post inoculation. Impression smears and samples were prepared from each harvested chorioallantoic membrane for identification of the pox virus using Agar gel precipitation and indirect immunofluorescent techniques. Both tests showed positive results in all examined samples in the form of precipitin lines or specific cytoplasmic fluorescent reaction, respectively. Gross examination of the infected udders primarily revealed, tenderness followed by embedded or superficial papule with eroded or ulcerated surfaces on the teats and lower, part of the udder. Histopathological study revealed focal infiltration of mononuclear and polymorphnuclear leucocytes in the dermal papillae with exocytosis of the inflammatory cells. Mild oedema and perivascular cuffing were also seen in the dermal layer. The cells of the stratum spinosum showed ballooning in addition to necrobiotc changes particularly near the ulcerative areas that was highly infiltrated by polymorphnuclear leucocytes. | ||||
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