Exploring the Cytoprotective Role of Liraglutide in Non-Alcoholic Fatty Liver Disease Experimentally Induced in the Rat Model (Histological and Biochemical Study) | ||
| Zagazig University Medical Journal | ||
| Article 7, Volume 30, Issue 9, December 2024, Pages 4348-4366 PDF (3.52 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zumj.2024.321384.3582 | ||
| Authors | ||
| Sahar F Shaban1; Dalia A Mohamed1; Aya Ismael Elsayed Mahmoud* 2; Heba Mohamed Abdel-aziz1 | ||
| 1Histology and Cell Biology Department, Faculty of Medicine, Zagazig University | ||
| 2Demonstrator of Histology and Cell Biology Faculty of Medicine, Suez University | ||
| Abstract | ||
| Background: Nonalcoholic fatty liver disease (NAFLD) is a widespread health issue and is considered the most common liver disease globally. Liraglutide, a glucagon-like peptide-1 receptor agonist, is a promising and innovative drug. Our study aimed to investigate the protective role of liraglutide in experimentally induced NAFLD in the rat model. Methods: Forty healthy adult male albino rats were used, they were assigned to 3 main groups: Group I (Control) which is subdivided into two equal subgroups, Group II (HFD group) which rats were given HFD for 12 weeks, and Group III (HFD+liraglutide) in which rats were fed with HFD in the same way as group II in concomitant with liraglutide that was injected subcutaneously daily (0.2 mg/kg) for 12 weeks. Blood samples and liver tissues were collected and assayed for biochemical, histological, and immunohistochemical studies. Results: The HFD group exhibited a significant increase in BMI, AC, serum ALT, AST, and serum lipid profile levels. Additionally, hepatic destructive changes were observed such as hepatocellular vacuolation, apoptosis, and congested blood vessels, along with a significant increase in the area percentage of collagen fibers, caspase 3 immunoreactions, and the number of GFAP immunoreactivity of hepatic stellate cells. These distortions were confirmed by ultrastructural assessment. In contrast, the HFD+liraglutide group exhibited normal BMI, AC, ALT, AST, and lipid profiles with critical preservation of the liver histoarchitecture. Conclusion: NAFLD significantly affects hepatic biochemical parameters and cytoarchitecture, leading to fibrosis and apoptosis. Liraglutide administration with a high-fat diet can protect the liver from these changes. | ||
| Keywords | ||
| NAFLD; Liraglutide; Caspase 3; GFAP | ||
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