SEMINAL PLASMA GLUTATHIONE PEROXIDASE IN RELATION TO FUNCTIONAL COMPETENCE OF CRYOPRFSERVED BUFFALO SPERMATOZOA | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 53, Issue 3, July 2005, Page 863-871 PDF (3.56 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2005.385419 | ||||
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| Authors | ||||
| M Waheed* 1; Mona Alaa Alaa El-Deen1; K El-Shahat2; T Khalifa3; A Ghalab2 | ||||
| 1Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University | ||||
| 2Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University | ||||
| 3Department of Biology, Animal Reproduction Research Institute, Al-Harm, Giza | ||||
| Abstract | ||||
| Glutathione peroxidase (GPx) has a central role in the defense against oxidative damage: however, data on GPx activity in the buffalo semen is limited. To expand this knowledge GPx activity in the seminal plasma of buffalo-bulls was studied. Fifty-five semen samples were collected from 19 buffalo-bulls and evaluated before cryopreservation. Aspartate aminotransferase (AST) level was assayed in the seminal plasma before freezing and after freezing- thawing. The results revealed GPx activity (14.59 ± 0.50 U/ml) in seminal plasma that varied with age of the buffalo-bulls. Older age was associated with a decrease in GPx activity. Increasing GPx activity in the seminal plasma was accompanied by a significant de-crease in sperm motility of freshly collected semen. The rate of increase in post-thaw sperm motility in response to 5 mM pentoxifylline was high in samples with high GPx activity before freezing. Increased GPx activity before freezing resulted in high percentages of abnormal mid-piece and tails post-thawing. Positive correlations existed between AST levels in the seminal plas-ma (before freezing and after thawing) and frequency of-post-thaw sperm cell abnormalities. | ||||
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