Phytochemical Profiling, Cytotoxicity and Anti-inflammatory Potential of Salvia hispanica L. Seeds | ||
| Egyptian Journal of Chemistry | ||
| Volume 67, Issue 12, December 2024, Pages 409-422 PDF (2.13 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2024.303803.10000 | ||
| Authors | ||
| Heba Raafat Mohamed* 1; Eman Ahmed El-Wakil2; Manal Mortady Hamed3; Zeinab Abd El-Khalek El-Shahid4 | ||
| 1Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Giza, Egypt | ||
| 2medicinal chemistry, theodor bilharz research institute | ||
| 3Medicinal Chemistry, Theodor Bilharz Research Institute, Giza, Egypt | ||
| 4Chemistry of Natural and Microbial Products Department, National research centre. | ||
| Abstract | ||
| Seeds of Salvia hispanica L. (chia seeds) have recently gained increasing attention as a functional food with numerous therapeutic benefits. The ethyl acetate and the methanol extracts of chia seeds were evaluated for their anti-inflammatory potential by measuring Nitric oxide production in LPS-stimulated RAW 264.7 cells and cytotoxic activity against HepG2, Paca II, HCT116, and HT29 cancer cell lines using MTT assay. The phytochemical composition was analyzed via GC-MS and UPLC-MS. Nineteen compounds were tentatively identified in the methanol extract via LC-MS and 10 compounds from the ethyl acetate extract by GC/MS. The ethyl acetate extract showed a remarkable cytotoxic effect against HepG2 and Paca-II with IC50 of 11 and 87.7 µg/mL, respectively. The pre-treatment with both extracts significantly suppressed the NO production in LPS-stimulated RAW 264.7 cells. The results indicated that; S. hispanica L. seeds could be used as a potent anti-inflammatory and anticancer herbal medicine especially against hepatocellular carcinoma. | ||
| Keywords | ||
| Salvia hispanica L; UPLC-ESI-MS; GC/MS; anticancer; anti-inflammatory | ||
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