Assessment of the Potential Effect of S-Methylcysteine Alone and in Combination with Metal-Organic Framework Nanoparticles on Experimental Toxoplasmosis in Immunosuppressed Mice
El-Sayed, S., Eassa, A., Ismail, M., Elmallawany, M., Abd Elhady, S., Abdelhameed, R., Barakat, A. (2024). Assessment of the Potential Effect of S-Methylcysteine Alone and in Combination with Metal-Organic Framework Nanoparticles on Experimental Toxoplasmosis in Immunosuppressed Mice. EKB Journal Management System, (), 1-12. doi: 10.21608/ejvs.2024.313962.2327
Shaimaa helmy El-Sayed; Ahmed Eassa; Mousa Ismail; Marwa Elmallawany; Sara Abd Elhady; Reda Abdelhameed; Ashraf Mohamed Barakat. "Assessment of the Potential Effect of S-Methylcysteine Alone and in Combination with Metal-Organic Framework Nanoparticles on Experimental Toxoplasmosis in Immunosuppressed Mice". EKB Journal Management System, , , 2024, 1-12. doi: 10.21608/ejvs.2024.313962.2327
El-Sayed, S., Eassa, A., Ismail, M., Elmallawany, M., Abd Elhady, S., Abdelhameed, R., Barakat, A. (2024). 'Assessment of the Potential Effect of S-Methylcysteine Alone and in Combination with Metal-Organic Framework Nanoparticles on Experimental Toxoplasmosis in Immunosuppressed Mice', EKB Journal Management System, (), pp. 1-12. doi: 10.21608/ejvs.2024.313962.2327
El-Sayed, S., Eassa, A., Ismail, M., Elmallawany, M., Abd Elhady, S., Abdelhameed, R., Barakat, A. Assessment of the Potential Effect of S-Methylcysteine Alone and in Combination with Metal-Organic Framework Nanoparticles on Experimental Toxoplasmosis in Immunosuppressed Mice. EKB Journal Management System, 2024; (): 1-12. doi: 10.21608/ejvs.2024.313962.2327

Assessment of the Potential Effect of S-Methylcysteine Alone and in Combination with Metal-Organic Framework Nanoparticles on Experimental Toxoplasmosis in Immunosuppressed Mice

Egyptian Journal of Veterinary Sciences
Articles in Press, Corrected Proof, Available Online from 16 October 2024    PDF (1.25 M)
Document Type: Original Article
DOI: 10.21608/ejvs.2024.313962.2327
Authors
Shaimaa helmy El-Sayed1; Ahmed Eassa2; Mousa Ismail2; Marwa Elmallawany2; Sara Abd Elhady* 3; Reda Abdelhameed4; Ashraf Mohamed Barakat5
1Medical Parasitology Department, Faculty of Medicine, Helwan University, Cairo, Egypt.
2Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt.
3Medical parasitology department, faculty of medicine, Helwan university
4Applied Organic Chemistry Department, National Research Centre, Giza, Egypt.
5Department of Zoonotic Diseases, Veterinary Research Institute, National Research Centre, 33 Bohouth str. Dokki, Giza, Egypt
Abstract
This study was conducted to investigate the potential therapeutic effect of S-methylcysteine (SMC) used singly, in combination with pyrimethamine (P) and sulfadiazine (S) regimen, and in conjugation with metal-organic framework nanoparticles (MOFs) on chronic toxoplasmosis in immunosuppressed mice. Fifty-four mice were immunosuppressed and divided into 9 groups (G); GІ (negative control), GП (positive control), GⅢ- GⅣf (infected and treated); GⅢ (P-S), GⅣa (SMC50), GⅣb (SMC25), GⅣc (SMC25, P-S), GⅣd (SMC25-MOFs), GⅣe (SMC25, P-MOFs, and S-MOFs), and GⅣf (SMC25-MOFs, P-MOFs, and S-MOFs). The infected mice were orally inoculated with 20 Toxoplasma cysts/ mouse. Mice treatment stared Fifty days post infection for 10 consecutive days. Assessment of the therapeutic effect of each treatment model included: parasitological parameters (brain cyst burden), histopathological examination of mice brain sections, and immunological assessment of mice serum interferon gamma (IFN-Υ) levels. GⅣf exhibited the highest percentage of brain cyst reduction (70.54%) and the highest percentage of IFN-Υ levels increase (182.23%). Nevertheless, significant increase in glial cell number, an evident increase in the degree of inflammation. Drugs loaded on MOFs have anti-Toxoplasma effect on chronic toxoplasmosis in immunosuppressed mice.
Keywords
T. gondii; Pyrimethamine; Sulfadiazine; SMC; MOFs
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