Molecular Characterization of Carbapenamases in Hypervirulent Klebsiella pneumoniae Isolates among Pediatric Patients | ||||
| Egyptian Journal of Medical Microbiology | ||||
| Article 13, Volume 34, Issue 1, January 2025, Page 111-118 PDF (570.92 K) | ||||
| Document Type: New and original researches in the field of Microbiology. | ||||
| DOI: 10.21608/ejmm.2024.322402.1342 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Lamiaa A. Salama1; Safaa M. Elageery  2; Nashwa M. Alkasaby2; Noha T. Abou El-Khier2; Iman M. Fawzy3; Mayada S. Zeid4; Dina F. Badr2
| ||||
| 1Microbiology and Immunology Department, Faculty of Pharmacy, Horus University, Egypt | ||||
| 2Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Egypt | ||||
| 3Mansoura Central Laboratories, Clinical Pathology Department, Ministry of Health, Egypt | ||||
| 4Infectious Diseases and Malnutrition Unit, Children Hospital, Faculty of Medicine, Mansoura University, Egypt | ||||
| Abstract | ||||
| Abstract Background: Carbapenem resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) have led to fatal outbreaks. The rapidly increasing detection rate of CR-hvKp in pediatric patients makes the diagnosis of CR-hvKp is critical to improve the clinical utilization of antibiotics. Aim: The present study aims at isolation and antibiotyping of CR-hvKp from clinical samples in Mansoura University Children Hospital. The study also directs to characterize carbapenemases in CR-hvKp isolates. Methodolgy: Eighty five K. pneumoniae isolates were tested for both carbapenems resistance and hypervirulence through detection of peg-344 gene by PCR. Antimicrobial susceptibility testing was done for CR-hvKp isolates by means of Kirby Bauer disc diffusion method. Multiplex polymerase chain reaction (multiplex PCR) was done to detect cabapenemase genes in CR-hvKp isolates. Results: Within the 85 K. pneumoniae isolates, 27strains (27/85; 31.76) were CR-Kp. Among CR-Kp isolates, 20 strains (20/27; 74.07%) were CR-hvKp. All CR-hvKp isolates were resistant to co-amoxiclav, piperacillin-tazobactam, cephalosporines and carbepenams, with variable resistant pattern to aminoglycosides, aztreonam, ciprofloxacin and levofloxacin. Respiratory samples were the main sample type of CR-hvKP isolates (60%), and tracheal intubation was the higher risk factor (50%). Cabapenemase genes were detected in only 13 isolates (13/20; 65%) . The identified carbapenemase genes included bla KPC-2 gene which was the predominant gene (6/13; 46.15%), bla VIM gene was present in 3 isolates (23.08%), and bla IMP and bla NDM genes each was present in 2 isolates (15.38% for each gene). Conclusion: It is critical to improve the clinical responsiveness and management of CR-hvKP infections, especially amongst pediatric patients. | ||||
| Keywords | ||||
| Klebsiella pneumoniae; hypervirulent; carbapenem resistant; pediatric patients | ||||
|
Statistics Article View: 347 PDF Download: 124 |
||||
