Induced Mutations in Gaillardia pulchella Foug Plants by Chemical Mutagen and Detection Variation by (SRAP) Sequence-Related Amplified Polymorphism Markers | ||||
| Journal of Agricultural Sciences and Sustainable Development | ||||
| Volume 1, Issue 4, December 2024, Page 389-404 PDF (1.29 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jassd.2024.299113.1029 | ||||
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| Authors | ||||
Ragab M.S. Radwan   1; Rawia A. Eid 2; Nahed G. Abd El-Aziz 3; Heba A. Mahfouze 4; M. A. El-Khateeb5; Hossam A. Ashour 5
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| 1Department of Ornamental Plants and Woody Trees Agricultural and Biological Research Institute, National Research Centre Dokki, Giza, Egypt | ||||
| 2Ornamental plants and woody trees department, Agricultural and biological research institute, National research center, Dokki, Giza, Egypt | ||||
| 3Ornamental Plants and Woody Trees Department, Institute of Agricultural and Biological Research’s, National Research Centre, Dokki, Cairo, Egypt | ||||
| 4Genetics and Cytology Department, Biotechnology Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt | ||||
| 5Department of Ornamental Hort., Faculty of Agric., Cairo University, Egypt | ||||
| Abstract | ||||
| Diethyl sulphate (DES) was used to induce genetic variability in Gaillardia pulchella plant to improving morphological characters. Besides, changes in the genomic DNA between mutant plants and the control were determined. The seeds were soaked in four different concentrations of DES (1000, 2000, 3000, and 4000 ppm) for 8 h. The results indicated that untreated plants gave the highest values of seed germination in M1 and M2, whilst 4000 ppm recorded the lowest value. The concentrations of 1000 and 2000 ppm increased the plant height, giving values of (125.47 and 118.96 cm in M1) and (120.10 and 121.45 cm in M2) compared to (110.07 cm in M1 and 114.12 cm in M2) for the control. The highest numbers of branches were (15.23 and 16.87 in M1) and (17.10 and 18.17 in M2) compared to (12.27 in M1 and 14.20 in M2) for the control. 3000 and 4000 ppm delayed flowering compared to the control. In contrast, 2000 ppm induced early flowering and increased the flowers number, compared to the control. Many mutants in leaves and inflorescences morphology were observed; the largest number of these mutants was obtained from 4000 ppm. SRAP mars were used to confirm the existence of genetic variability at the genomic DNA level among populations treated with DES and control, depending on the concentration of DES. Therefore, SRAP markers are considered an important tool in detecting the mutagenic effects of DES. Also, it will help to discriminate between populations showing mutations in morphological and floral characteristics. | ||||
| Keywords | ||||
| Mutations; Gaillardia pulchella; DES; vegetative growth; flowering; polymorphism; SRAP markers | ||||
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