Targeting Sarcocystis fusiformis cysteine protease as a possible approach to limit sarcocystosis infection
Barakat, A., Abdel-Aty, A., Salah, H., Ibrahim, M., Ibrahim, T., Shaapan, R., Mohamed, S. (2024). Targeting Sarcocystis fusiformis cysteine protease as a possible approach to limit sarcocystosis infection. EKB Journal Management System, 67(13), 911-924. doi: 10.21608/ejchem.2024.325966.10587
Amal Z. Barakat; Azza M. Abdel-Aty; Hala A. Salah; Marwa K. Ibrahim; Taghreed B. Ibrahim; Raafat M Shaapan; Saleh A. Mohamed. "Targeting Sarcocystis fusiformis cysteine protease as a possible approach to limit sarcocystosis infection". EKB Journal Management System, 67, 13, 2024, 911-924. doi: 10.21608/ejchem.2024.325966.10587
Barakat, A., Abdel-Aty, A., Salah, H., Ibrahim, M., Ibrahim, T., Shaapan, R., Mohamed, S. (2024). 'Targeting Sarcocystis fusiformis cysteine protease as a possible approach to limit sarcocystosis infection', EKB Journal Management System, 67(13), pp. 911-924. doi: 10.21608/ejchem.2024.325966.10587
Barakat, A., Abdel-Aty, A., Salah, H., Ibrahim, M., Ibrahim, T., Shaapan, R., Mohamed, S. Targeting Sarcocystis fusiformis cysteine protease as a possible approach to limit sarcocystosis infection. EKB Journal Management System, 2024; 67(13): 911-924. doi: 10.21608/ejchem.2024.325966.10587

Targeting Sarcocystis fusiformis cysteine protease as a possible approach to limit sarcocystosis infection

Egyptian Journal of Chemistry
Volume 67, Issue 13, December 2024, Pages 911-924    PDF (1.62 M)
Document Type: Original Article
DOI: 10.21608/ejchem.2024.325966.10587
Authors
Amal Z. Barakat1; Azza M. Abdel-Aty1; Hala A. Salah1; Marwa K. Ibrahim2; Taghreed B. Ibrahim3; Raafat M Shaapan4; Saleh A. Mohamed* 1
1Molecular Biology Department, National Research Centre, Dokki, Cairo, Egypt
2Department of Microbial Biotechnology, National Research Centre, Dokki, Cairo, Egypt
3Department of Hydrobiology, National Research Centre, Dokki, Cairo, Egypt
4Department of Zoonotic Diseases , Veterinary Research Division, National Research Center, Dokki, Cairo, Egypt
Abstract
Worldwide, sarcocystosis infection in Egyptian buffaloes is common and lack a particular therapy. A parasite cysteine protease (CP) is involved in several biological and pathogenetic activities, including the parasites' adhesion to host cells, tissue invasion, cytotoxicity, intake of nutrients, and immune evasion. We have previously reported the sequence of purified cysteine protease from S. fusiforms cysts. Herein, the purified S. fusiformis cysteine protease (SFCP) is used as an immunogenic for antisera production. Immunization with the SFCP-antigen promoted the immune response in rabbits and rats, exhibiting elevated specific antibodies. Thus, the therapeutic potential of the prepared antisera and SFCP vaccination was evaluated by employing S. fusiformis-infected rats. The result demonstrated that SFCP and the prepared antisera alleviate the oocyst shedding and intestinal damage in S. fusiformis infected animals. The serum levels of Th1 cytokines, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ and interleukin (IL)-12 as well as the Th2 cytokines (IL-4 and IL-6) were significantly increased in infected rats, with a maximum increase by ~1.75- 2.45 at week 2 post-infection compared to the control. Additionally, mRNA levels of nucleotide-binding oligomerization domain (NOD)1, NOD2, TNF-α, TLR4,TLR9, IFN-γ, IL-10,IL-6, transforming growth factor-beta (TGF-β) and IL-β were all up-regulated in the infected group compared to the control. However, the SFCP-vaccination and anti-SFCP sera modulate these elevations in serum and spleen. These findings demonstrated that the SFCP-vaccination and anti-SFCP sera could be novel therapeutic candidates for controlling S. fusiformis infection and its harmful effects.
Keywords
Sarcocystis fusiformis; Cysteine protease; Vaccination, Antisera; Cytokines
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